Role of the Mevalonate Pathway in Adrenocortical Tumorigenesis
| Autor: | Helena Panteliou Lima-Valassi, Luciana Ribeiro Montenegro, Berenice B. Mendonca, Madson Q. Almeida, Maria Candida Barisson Villares Fragoso, Chin Jia Lin, Antonio M. Lerario |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Carcinogenesis Cell Survival Endocrinology Diabetes and Metabolism Clinical Biochemistry Protein Prenylation Mevalonic Acid medicine.disease_cause Biochemistry Young Adult chemistry.chemical_compound Endocrinology Cell Line Tumor Internal medicine medicine Humans Viability assay Aged Cholesterol Cell growth Cholesterol side-chain cleavage enzyme Biochemistry (medical) Infant General Medicine Middle Aged Adrenal Cortex Neoplasms Neoplasm Proteins Gene Expression Regulation Neoplastic stomatognathic diseases chemistry CYP17A1 Child Preschool HSD3B1 Cancer research Female Steroids lipids (amino acids peptides and proteins) Mevalonate pathway Metabolic Networks and Pathways |
| Zdroj: | Hormone and Metabolic Research. 53:124-131 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/a-1322-2943 |
| Popis: | 3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is the rate-limiting enzyme of the mevalonate pathway, which generates cholesterol and non-sterol compounds such as isoprenoid, which are involved in key steps of tumorigenesis such as cell growth and proliferation. Our aim was to evaluate the role of the mevalonate pathway in adrenocortical tumors (ACTs). Expression pattern of HMGCR, FDFT1, LDLR, SCARB1, StAR, TSPO, CYP11A1, CYP11B1, CYP17A1, CYP21A1, and HSD3B1 genes, involved in the mevalonate pathway and steroidogenesis, was quantified by real-time RT-PCR in 46 ACT [14 adenomas (ACA) and 11 carcinomas (ACC) from adults and 13 ACA and 8 ACC from pediatric patients]. Effects of the mevalonate pathway inhibition on NCI-H295A cell viability was assessed by colorimetric assay. HMGCR was overexpressed in most adult ACT. The expression of TSPO, STAR, CYP11B1, CYP21A1, and HSD3B1 in adult ACC was significantly lower than in ACA (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|