In vitrotoxicological assessment of iron oxide, aluminium oxide and copper nanoparticles in prokaryotic and eukaryotic cell types
| Autor: | Ameena Mobeen, Rakhshinda Sadiq, Qaiser M. Khan, Amer Jamal Hashmat |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
DNA damage Health Toxicology and Mutagenesis Binucleated cells Colony Count Microbial Metal Nanoparticles Kidney Toxicology medicine.disease_cause Ferric Compounds Cell Line Microbiology Ames test Aluminum Oxide medicine Animals Cytotoxicity Escherichia coli Pharmacology Micronucleus Tests Chemical Health and Safety Bacteria Dose-Response Relationship Drug Mutagenicity Tests Chemistry DNA Breaks technology industry and agriculture Public Health Environmental and Occupational Health Haplorhini General Medicine Comet assay Biochemistry Micronucleus test Comet Assay Copper Genotoxicity DNA Damage |
| Zdroj: | Drug and Chemical Toxicology. 38:152-161 |
| ISSN: | 1525-6014 0148-0545 |
| DOI: | 10.3109/01480545.2014.919584 |
| Popis: | Metallic nanoparticles (NPs) have a variety of applications in different industries including pharmaceutical industry where these NPs are used mainly for image analysis and drug delivery. The increasing interest in nanotechnology is largely associated with undefined risks to the human health and to the environment. Therefore, in the present study cytotoxic and genotoxic effects of iron oxide, aluminium oxide and copper nanoparticles were evaluated using most commonly used assays i.e. Ames assay, in vitro cytotoxicity assay, micronucleus assay and comet assay. Cytotoxicity to bacterial cells was assessed in terms of colony forming units by using Escherichia coli (gram negative) and Bacillus subtilis (gram positive). Ames assay was carried out using two bacterial strains of Salmonella typhimurium TA98 and TA100. Genotoxicity of these NPs was evaluated following exposure to monkey kidney cell line, CHS-20. No cytotoxic and genotoxic effects were observed for iron oxide, and aluminium oxide NPs. Copper NPs were found mutagenic in TA98 and in TA100 and also found cytotoxic in dose dependent manner. Copper NPs induced significant (p |
| Databáze: | OpenAIRE |
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