Analysis of retinal and 3-dehydroretinal in the retina by high-pressure liquid chromatography
| Autor: | Momoyo Makino-Tasaka, Tatsuo Suzuki |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
genetic structures
Kinetics Biophysics Biochemistry High-performance liquid chromatography Retina 3-dehydroretinal Pigment chemistry.chemical_compound Species Specificity Bullfrog Decapoda Goldfish Oximes medicine Animals Vitamin A Molecular Biology Chromatography High Pressure Liquid Rana catesbeiana Chromatography Chemistry Retinal Cell Biology Chromophore medicine.anatomical_structure visual_art Retinaldehyde visual_art.visual_art_medium sense organs Retinal Pigments |
| Zdroj: | Analytical Biochemistry. 129:111-119 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(83)90059-3 |
| Popis: | A sensitive analytical method was developed in order to study the rhodopsin-porphyropsin system in the eye. Oximes of 11-cis-retinal, all-trans-retinal, 11-cis-3-dehydroretinal, and all-trans-3-dehydroretinal were determined quantitatively by high-pressure liquid chromatography. This method was applied to the analysis of retinal and 3-dehydroretinal in the retinas of bullfrog and goldfish. The results agreed with those obtained from the bleaching kinetics of visual pigment extracted with detergent. A reliable result is obtained if the tissue contains more than 5 pmol of retinal (or 3-dehydroretina). The chromophore composition could be determined in the eye of a small freshwater prawn, Palaemon pancidence, using 50 pmol of 11-cis-retinal and no 3-dehydroretinal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|