Isobutyrylshikonin and isovalerylshikonin from the roots of Onosma visianii inhibit larval growth of the tobacco cutworm Spodoptera littoralis
| Autor: | Roman Pavela, Stefano Dall'Acqua, Giovanni Benelli, Vladislav Kolarčik, Stefania Sut, Giulio Lupidi, Filippo Maggi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Aché H NMR Biology Acetyl cholinesterase (AChE) Botanical insecticide Onosma visianii Clem Shikonin derivatives Spodoptera littoralis 01 natural sciences Median lethal dose Cutworm Lepidoptera genitalia Column chromatography Botany Traditional medicine 010405 organic chemistry Boraginaceae biology.organism_classification language.human_language 0104 chemical sciences language Noctuidae Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Industrial Crops and Products. 109:266-273 |
| ISSN: | 0926-6690 |
| DOI: | 10.1016/j.indcrop.2017.08.048 |
| Popis: | In the present work, we evaluated the toxicity and growth inhibitory effects on Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) of the root hexane extract from Onosma visianii (Boraginaceae). Furthermore, two naphtooquinone derivatives, namely isobutyrylshikonin and isovalerylshikonin, which were isolated from the extract by column chromatography and identified by Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) techniques, were also assayed for the same target. The O. visianii extract, at 30.0 μg/cm 2 , led to 100% mortality at day 7, with LD 50 of 6.8 μg/cm 2 for the same day. On the other hand, isovalerylshikonin was significantly more effective (LD 50 = 0.8 μg/cm 2 ) than isobutyrylshikonin (LD 50 = 7.3 μg/cm 2 ). The two tested naphtoquinones inhibited the growth of S. littoralis larvae with ED 50 of 0.4 and 0.3 μg/cm 2 , respectively. Furthermore, isovalerylshikonin provide a stronger inhibition of acetylcholinesterase (AChE) enzyme (IC 50 = 51.4 μg/mL), than isobutyrylshikonin (IC 50 = 162.0 μg/mL). These findings provided new insights into the potential of shikonin derivatives as new valuable botanical insecticides to be used in crop protection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect