| Autor: |
Worsley, Sarah F., Innocent, Tabitha M., Holmes, Neil A., Al-Bassam, Mahmoud M., Schiøtt, Morten, Wilkinson, Barrie, Murrell, J. Colin, Boomsma, Jacobus J., Yu, Douglas W., Hutchings, Matthew I. |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.16625251 |
| Popis: |
Additional file 1: Supplemental tables, figures and methods referenced in the text. Fig. S1. Overview of methodology used for RNA stable isotope probing; Fig. S2. Fluorescent microscopy images of glucose water diet treatment; Fig. S3. The relationship between RNA-SIP fraction number and buoyant density; Fig. S4. 16S rRNA gene copy number across different fractions of buoyant density gradients, as determined via qPCR; Fig. S5. The atom percentage of 13C in ants, as determined by Isotope Ratio Mass Spectrometry (IRMS) analysis; Fig. S6. The expression of Kegg orthology pathway categories in Pseudonocardia symbiont strains; Fig. S7. The bioactivity of Pseudonocardia isolates against the specialized fungus-garden pathogen Escovopsis weberi; Fig. S8. Individual growth-rate experiments of Acromyrmex-resident, non-producer strains; Fig. S9. Antibiotic resistance profiles for producer, non-producer, and resident non-producer strains; Table S1. Details of ant colonies, bacterial and fungal strains, reference genomes and primers used in experiments; Table S2. Details RNA-sequencing reads from ant propleural plate samples; Table S3. Secondary metabolite BGCs in the Pseudonocardia mutualist genomes (table adapted from [32]); Table S4. Media recipes and antibiotics used in this study. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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