Multicenter Evaluation of Rapid BACpro® II for the Accurate Identification of Microorganisms Directly from Blood Cultures Using MALDI-TOF MS
Autor: | Gilbert Greub, Anne Katrine Steffensen, Belén Rodríguez-Sánchez, David Rodriguez-Temporal, André Ingebretsen, Germán Bou, Lidia Quiroga, Antony Croxatto, Marina Oviaño, Guy Prod'hom |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
MALDI-TOF
Medicine (General) Gram-negative bacteria Chromatography biology medicine.diagnostic_test Chemistry Microorganism Gram-positive bacteria Clinical Biochemistry Gold standard (test) rapid BACpro® II kit mass spectrometry blood culture rapid identification biology.organism_classification Matrix-assisted laser desorption/ionization R5-920 Species level medicine Blood culture Gram |
Zdroj: | Diagnostics; Volume 11; Issue 12; Pages: 2251 Diagnostics, Vol 11, Iss 2251, p 2251 (2021) |
ISSN: | 2075-4418 |
DOI: | 10.3390/diagnostics11122251 |
Popis: | The identification of microorganisms directly from blood cultures using MALDI-TOF MS has been shown to be the most impacting application of this methodology. In this study, a novel commercial method was evaluated in four clinical microbiology laboratories. Positive blood culture samples (n = 801) were processed using a rapid BACpro® II kit and then compared with the routine gold standard. A subset of monomicrobial BCs (n = 560) were analyzed in parallel with a Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) and compared with the rapid BACpro® II kit. In addition, this kit was also compared with two different in-house methods. Overall, 80.0% of the monomicrobial isolates (609/761; 95% CI 71.5–88.5) were correctly identified by the rapid BACpro® II kit at the species level (92.3% of the Gram negative and 72.4% of the Gram positive bacteria). The comparison with the Sepsityper® Kit showed that the rapid BACpro® II kit generated higher rates of correct species-level identification for all categories (p > 0.0001), except for yeasts identified with score values > 1.7. It also proved superior to the ammonium chloride method (p > 0.0001), but the differential centrifugation method allowed for higher rates of correct identification for Gram negative bacteria (p > 0.1). The percentage of accurate species-level identification of Gram positive bacteria was particularly noteworthy in comparison with other commercial and in-house methods. |
Databáze: | OpenAIRE |
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