Alleles and genotypes of polimorphisms of IL-18, TNF-α and IFN-γ are associated with a higher risk and severity of hepatocellular carcinoma (HCC) in Brazil
| Autor: | Eduardo Antônio Donadi, Valdair Francisco Muglia, Andreza Correa Teixeira, Jorge Elias-Junior, Ana de Lourdes Candolo Martinelli, N. H. S. Deghaide, Celso T. Mendes-Junior, Marie Secaf, Leonardo Arduino Marano |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Risk Pathology medicine.medical_specialty Carcinoma Hepatocellular Adolescent Genotype Immunology Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Metastasis Interferon-gamma Young Adult Gene Frequency Polymorphism (computer science) medicine Carcinoma Odds Ratio Immunology and Allergy Humans Genetic Predisposition to Disease Allele Allele frequency Alleles Aged Retrospective Studies Tumor Necrosis Factor-alpha Liver Neoplasms Interleukin-18 General Medicine Middle Aged medicine.disease Molecular biology Cross-Sectional Studies INTERLEUCINAS Hepatocellular carcinoma Case-Control Studies Female Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Hepatocellular carcinoma (HCC) is a primary malignant tumor of the liver. We evaluated the association of alleles and genotypes of polymorphisms of IL-18 (-607C/A and -137G/C), IFN-γ (+874T/A) and TNF-α (-238G/A and -308G/A) with the risk and severity of HCC. One-hundred-and-twelve patients with HCC and 202 healthy controls were studied. Single nucleotide polymorphisms (SNPs) were amplified by PCR with specific primers and the products were submitted to polyacrylamide gel electrophoresis and stained with silver. We evaluated tumor presentation, tumor size and presence of metastasis. Significant higher risk of HCC was associated with: alleles IL-18 -607(*)A (P=0.0235; OR=1.48; 95%CI=1.06-2.08); TNF-α -238(*)A (P=0.0025; OR=2.12; 95%CI=1.32-3.40) and TNF-α -308(*)A (P=0.0351; OR=1.82; 95%CI=1.07-3.08); and genotypes IL-18-607AA (P=0.0048; OR=3.03; 95%CI=1.40-6.55); TNF-α -238GA (P=0.0011; OR=2.44; 95%CI=1.45-4.12); and TNF-α -308GA (P=0.0031; OR=2.51; 95%CI=1.39-4.51). Significant association was found between multinodular HCC and IL-18 -607(*)C allele (P=0.029; OR=2.40, 95%CI: 1.09-5.28), and IL-18 -607CC genotype (P=0.028; OR=3.5, 95%CI: 1.24-9.86). Diffuse HCC was significantly associated with IFN-γ +874TA genotype (P=0.044; OR=3.6, 95%CI: 1.03-12.47). The IL-18 -137(∗)C allele showed a significant association with the presence of metastasis. Thus, IL-18 -607(*)A and TNF-α (-238(*)A and -308(*)A) alleles may confer susceptibility to HCC, while IL-18 -607(*)C and -137(*)C alleles more severe disease. |
| Databáze: | OpenAIRE |
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