Eugenol prevents fMLF-induced superoxide anion production in human neutrophils by inhibiting ERK1/2 signaling pathway and p47phox phosphorylation
| Autor: | Amina Chniguir, Rafik Bachoual, Dan Liu, Coralie Pintard, Pham My-Chan Dang, Jamel El-Benna |
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| Přispěvatelé: | Faculty of Sciences of Gabès, Université de Gabès, Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Centre de recherche biomédicale Bichat-Beaujon (CRB3), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Gabes |
| Rok vydání: | 2019 |
| Předmět: |
MAP Kinase Signaling System
Neutrophils p38 mitogen-activated protein kinases Primary Cell Culture lcsh:Medicine [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Antioxidants Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Superoxides Eugenol Humans Phosphorylation lcsh:Science IC50 ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Multidisciplinary NADPH oxidase biology Chemistry Superoxide Cytochrome c Chemotaxis Chronic obstructive pulmonary disease lcsh:R NADPH Oxidases Chronic inflammation Molecular biology Healthy Volunteers 3. Good health Blot N-Formylmethionine Leucyl-Phenylalanine Oxidative Stress 030220 oncology & carcinogenesis biology.protein [SDV.IMM]Life Sciences [q-bio]/Immunology lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-019-55043-8⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-55043-8⟩ |
| Popis: | Eugenol is a polyphenol extracted from Syzygium aromaticum essential oil. It is known to have anti-inflammatory and chemoprotective properties as well as a potent anti-oxidant activity due the presence of its phenolic group. In this study, we examined the effects of eugenol on neutrophil superoxide production, a key process involved in innate immunity and inflammation. Superoxide anion generationin human neutrophils was measured by cytochrome c reduction assay. Western blotting was used to analyze the phosphorylation of, p47phox, MAPKinases (p38 and ERK1/2), MEK1/2 and Raf, key proteins involved in the activation of NADPH oxidase. Pretreatment of neutrophils by increasing concentrations (2.5 µg/mL–20 µg/mL) of eugenol for 30 min, inhibited significantly (p − production was affected only at the highest eugenol concentration (20 µg/mL). Results showed that eugenol decreased the phosphorylation of p47phox onSer-345 and Ser-328, the translocation of p47phox to the membranesand the phosphorylation of Raf, MEK1/2 and ERK1/2 proteins. Taken together, our results suggest that eugenol inhibits the generation of superoxide anion by neutrophils via the inhibition of Raf/MEK/ERK1/2/p47phox-phosphorylation pathway. |
| Databáze: | OpenAIRE |
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