Glycoconjugates ofTrypanosoma cruzi: A 74 kD antigen of trypomastigotes specifically reacts with lytic anti-α-galactosyl antibodies from patients with chronic Chagas disease
| Autor: | Antoniana U. Krettli, Igor C. Almeida, Greice M. Krautz, Luiz R. Travassos |
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| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Chagas disease Glycoconjugate Trypanosoma cruzi Molecular Sequence Data Clinical Biochemistry Antibodies Protozoan Oligosaccharides Heterologous Antigens Protozoan Enzyme-Linked Immunosorbent Assay Cross Reactions Biology Antigen medicine Animals Humans Immunology and Allergy Chagas Disease chemistry.chemical_classification Biochemistry (medical) Public Health Environmental and Occupational Health Galactose Hematology medicine.disease biology.organism_classification Virology Molecular Weight Medical Laboratory Technology Chronic infection Carbohydrate Sequence chemistry Lytic cycle Chronic Disease Luminescent Measurements biology.protein Antibody Glycoconjugates |
| Zdroj: | Journal of Clinical Laboratory Analysis. 7:307-316 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.1860070603 |
| Popis: | Protective, lytic antibodies are believed to be correlated with active Trypanosoma cruzi infection. In patients with chronic infection, antibodies lysing trypomastigote forms recognize chiefly alpha-galactosyl structures at the parasite surface. The target molecules on cell-derived trypomastigotes that react with anti-alpha-galactosyl antibodies (anti-Gal) from patients with chronic Chagas disease were investigated. Glycoconjugates were isolated from trypomastigotes and shown to absorb purified Chagasic (Ch) anti-Gel effectively as well as lytic antibodies from Ch sera. Active fractions were F2 (74 kD and 95.6 kD) and F3 (120-200 kD). A differential reactivity with antibodies from untreated Ch patients (trypanolytic) and from treated, presumably cured, individuals (not trypanolytic) was evident using F2 and F3 antigenic fractions. No cross-reactivity with heterologous sera (other infections) was observed. The F2 glycoconjugate (mostly 74 kD) can be used in the diagnosis of active Chagas infection, replacing the quantitative determination of complement-mediated lysis. With the present sample of patients' sera and normal human sera, it showed 100% sensitivity and specificity. |
| Databáze: | OpenAIRE |
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