Characterization, isolation and cloning of sugarcane genes related to drought stress
| Autor: | Larissa Mara de Andrade, Thiago Romanos Benatti, Jorge da Silva, Silvana Creste, Antonio Figueira, Michael dos Santos Brito, Maria Helena S. Goldman, Paula Macedo Nobile, Ana Lilia Alzate Marin |
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| Rok vydání: | 2014 |
| Předmět: |
Abiotic stress
business.industry Jasmonic acid fungi Drought tolerance food and beverages General Medicine Genetically modified crops General Biochemistry Genetics and Molecular Biology Crop chemistry.chemical_compound chemistry Poster Presentation Botany Plant defense against herbivory Medicine Sugar business Gene |
| Zdroj: | BMC Proceedings |
| ISSN: | 1753-6561 |
| Popis: | Background Sugarcane is a major crop worldwide as raw material for sugar and ethanol production. Drought is one of the most limiting factor that affects sugarcane productivity. In order to understand the mechanisms of drought response, field and greenhouse assays were conducted with two droughtcontrasting sugarcane genotypes (IACSP94-2094/tolerant and IACSP97-7065/sensitive), and several genes up/downregulated under drought stress identified by microarrays and RNAseq analyses. Ten differential expressed genes in both assays were evaluated by qPCR [1], and three of them showed the transcriptional profile related drought tolerance: aLipoxygenase (ScLOX), acting in the biosynthesis of the jasmonic acid precursor, and recent studies showed their role in defense against drought stress [3]; bDehydrin, correlated to drought stress and associated to maintenance of turgor cells, [2]; cDirigent-jacalin, associated to resistance disease and abiotic stress tolerance [4] and also related to jasmonic acid, an important hormone on plant defense. These genes were chosen as target for functional analyses in rice and sugarcane transgenic plants. |
| Databáze: | OpenAIRE |
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