Matrix-based gene delivery for tissue repair
| Autor: | Tatiana Segura, Cynthia Cam |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Scaffold
Transgene Genetic Vectors Immobilized Nucleic Acids Biomedical Engineering Bioengineering Gene delivery Biology Transfection Article Absorption Paracrine signalling Animals Humans Transgenes Autocrine signalling Gene Wound Healing Gene Transfer Techniques DNA Genetic Therapy Molecular biology Cell biology Wound healing Biotechnology |
| Zdroj: | Current Opinion in Biotechnology. 24:855-863 |
| ISSN: | 0958-1669 |
| DOI: | 10.1016/j.copbio.2013.04.007 |
| Popis: | Scaffolds for tissue repair must provide structural and biochemical cues to initiate the complex cascade of events that lead to proper tissue formation. Incorporating genes into these scaffolds is an attractive alternative to protein delivery since gene delivery can be tunable to any DNA sequence and genes utilize the cells' machinery to continuously produce therapeutic proteins, leading to longer lasting transgene expression and activation of autocrine and paracrine signaling that are not activated with bulk protein delivery. In this review, we discuss the importance of scaffold design and the impact of its design parameters (e.g. material, architecture, vector incorporation, biochemical cue presentation) on transgene expression and tissue repair. |
| Databáze: | OpenAIRE |
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