Kinetic analysis of Streptococcus sanguis adhesion to artificial pellicle
| Autor: | K.G. Taylor, Ronald J. Doyle, M M Cowan |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
0301 basic medicine
Surface Properties Kinetics Kinetic energy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Adsorption Reaction rate constant Desorption Dental Deposits Humans Dental Pellicle Binding site Saliva General Dentistry Binding Sites Chemistry Adhesiveness 030206 dentistry Adhesion Hydroxylapatite Crystallography 030104 developmental biology Durapatite Models Chemical Biophysics Hydroxyapatites Streptococcus sanguis |
| Zdroj: | Journal of dental research. 65(10) |
| ISSN: | 0022-0345 |
| Popis: | Studies of equilibria between Streptococcus sanguis and artificial pellicle have suggested that there are multiple binding sites for the organism. In the present study, adhesion of S. sanguis to saliva-coated hydroxylapatite was examined by means of kinetic methods. Cell-pellicle complex formation was measured from initiation of binding to equilibrium. Rate constants were calculated for forward reactions (adsorption) and reverse reactions (desorption). Initial binding obeyed reversible, first-order kinetics, whereas desorption of bound cells followed biphasic kinetics. Initial desorption proceeded approximately ten times faster than the slower second rate. The results are consistent with the mechanism C + P reversible CP* in equilibrium with CP in which CP* represents the reversible equilibrium that shifts at a discrete rate to the high-affinity CP state. Thus, the biphasic binding behavior that has been previously deduced from equilibrium studies may be attributed to a time-dependent shift from close apposition to pellicle, stabilized by low-specificity forces, to a higher-affinity binding. |
| Databáze: | OpenAIRE |
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