Evaluation of Colilert-18 for the detection of coliforms and Escherichia coli in tropical fresh water
| Autor: | W.L. Chao |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Bacteriological Techniques
Tropical Climate food.ingredient Colony Count Microbial Fresh Water Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Agar food chemistry Fresh water medicine Colony count Escherichia coli False Positive Reactions Food science Analysis method Fatty acid methyl ester Environmental Monitoring |
| Zdroj: | Letters in applied microbiology. 42(2) |
| ISSN: | 0266-8254 |
| Popis: | Aims: To evaluate the suitability of Colilert-18 in detecting Escherichia coli and total coliforms in tropical freshwater samples. Methods and Results: Target organisms were isolated from yellow-fluorescent and yellow wells of Colilert-18/Quanti-Tray using m-TEC agar and m-ENDO LES agar respectively. All the selected isolates were first identified based on their fatty acid methyl ester profile. Isolates showing contradictory results to that of the Colilert-18 procedure were re-identified using API 20E strips. A total of 357 isolates, 177 from yellow-fluorescent wells and 180 from yellow wells, were identified. Conclusions: The false-positive and -negative rates for E. coli detection using Colilert-18 were 36·4% and 11%, respectively, while for coliform detection the false-positive rate was 10·3%. Significance and Impact of the Study: The high false-positive rate of Colilert-18, tempers its value for E. coli detection when used for tropical freshwater samples. |
| Databáze: | OpenAIRE |
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