Combining SIMS and mechanistic modelling to reveal nutrient kinetics in an algal-bacterial mutualism
| Autor: | Smith, Croze, Whitehouse, Foster, Peaudecerf, Laeverenz Schlogelhofer, Bunbury |
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| Přispěvatelé: | Laeverenz Schlogelhofer, Hannah [0000-0003-2958-5711], Peaudecerf, François J [0000-0003-0295-4556], Croze, Ottavio A [0000-0003-1906-0532], Apollo - University of Cambridge Repository, Peaudecerf, François J. [0000-0003-0295-4556], Croze, Ottavio A. [0000-0003-1906-0532] |
| Rok vydání: | 2021 |
| Předmět: |
Glycerol
Composite Particles Chlamydomonas reinhardtii Bacterial growth Nutrient Isotopes Microbial Physiology Mutualism (biology) 0303 health sciences Multidisciplinary biology Chemistry Axenic Culture Physics Monomers Mesorhizobium Microbial Growth and Development Eukaryota Plants Physical sciences Vitamin B 12 Experimental Organism Systems Environmental chemistry Medicine Biological Cultures Cell Culturing Techniques Research Article Optimization Biogeochemical cycle Atoms Algae Science Heterotroph FOS: Physical sciences Photosynthesis Microbiology Models Biological 03 medical and health sciences Model Organisms Plant and Algal Models Particle Physics Symbiosis 030304 developmental biology Bacteria Biology and life sciences 030306 microbiology Bacterial Growth Organisms biology.organism_classification Polymer Chemistry Mesorhizobium loti Research and analysis methods Mutation Animal Studies Mathematics Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 5, p e0251643 (2021) PLoS ONE, 16 (5) |
| DOI: | 10.17863/cam.70212 |
| Popis: | Funder: Raymond and Beverly Sackler Scholarship Funder: Mines ParisTech Funder: Swedish Museum of Natural History Funder: University of Iceland Funder: Consortium of Danish geoscience institutions Microbial communities are of considerable significance for biogeochemical processes, for the health of both animals and plants, and for biotechnological purposes. A key feature of microbial interactions is the exchange of nutrients between cells. Isotope labelling followed by analysis with secondary ion mass spectrometry (SIMS) can identify nutrient fluxes and heterogeneity of substrate utilisation on a single cell level. Here we present a novel approach that combines SIMS experiments with mechanistic modelling to reveal otherwise inaccessible nutrient kinetics. The method is applied to study the onset of a synthetic mutualistic partnership between a vitamin B12-dependent mutant of the alga Chlamydomonas reinhardtii and the B12-producing, heterotrophic bacterium Mesorhizobium japonicum, which is supported by algal photosynthesis. Results suggest that an initial pool of fixed carbon delays the onset of mutualistic cross-feeding; significantly, our approach allows the first quantification of this expected delay. Our method is widely applicable to other microbial systems, and will contribute to furthering a mechanistic understanding of microbial interactions. |
| Databáze: | OpenAIRE |
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