Gene expression profiling identifies pathways involved in seed maturation of Jatropha curcas
| Autor: | Tamás Deák, Stephan Pabinger, Margit Laimer, Hakim Tafer, Klemens Vierlinger, Fatemeh Maghuly |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:QH426-470
lcsh:Biotechnology Jatropha Biology High-throughput quantitative real-time PCR Microarray Transcriptome Biofuel Gene Expression Regulation Plant Next generation sequencing lcsh:TP248.13-248.65 Genetics Oligonucleotide Array Sequence Analysis Plant Proteins Phenylpropanoid Microarray analysis techniques Sequence Analysis RNA Gene Expression Profiling Gene Expression Regulation Developmental food and beverages Molecular Sequence Annotation biology.organism_classification Gene expression profiling lcsh:Genetics Flavonoid biosynthesis Metabolic pathways Seeds Gene expression DNA microarray Jatropha curcas Biotechnology Research Article |
| Zdroj: | BMC Genomics, Vol 21, Iss 1, Pp 1-19 (2020) BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Jatropha curcas, a tropical shrub, is a promising biofuel crop, which produces seeds with high content of oil and protein. To better understand the maturation process of J. curcas seeds and to improve its agronomic performance, a two-step approach was performed in six different maturation stages of seeds: 1) generation of the entire transcriptome of J. curcas seeds using 454-Roche sequencing of a cDNA library, 2) comparison of transcriptional expression levels using a custom Agilent 8x60K oligonucleotide microarray. Results A total of 793,875 high-quality reads were assembled into 19,382 unique full-length contigs, of which 13,507 could be annotated with Gene Ontology (GO) terms. Microarray data analysis identified 9111 probes (out of 57,842 probes), which were differentially expressed between the six maturation stages. The expression results were validated for 75 selected transcripts based on expression levels, predicted function, pathway, and length. Result from cluster analyses showed that transcripts associated with fatty acid, flavonoid, and phenylpropanoid biosynthesis were over-represented in the early stages, while those of lipid storage were over-represented in the late stages. Expression analyses of different maturation stages of J. curcas seed showed that most changes in transcript abundance occurred between the two last stages, suggesting that the timing of metabolic pathways during seed maturation in J. curcas occurs in late stages. The co-expression results showed that the hubs (CB5-D, CDR1, TT8, DFR, HVA22) with the highest number of edges, associated with fatty acid and flavonoid biosynthesis, are showing a decrease in their expression during seed maturation. Furthermore, seed development and hormone pathways are significantly well connected. Conclusion The obtained results revealed differentially expressed sequences (DESs) regulating important pathways related to seed maturation, which could contribute to the understanding of the complex regulatory network during seed maturation with the focus on lipid, flavonoid and phenylpropanoid biosynthesis. This study provides detailed information on transcriptional changes during J. curcas seed maturation and provides a starting point for a genomic survey of seed quality traits. The results highlighted specific genes and processes relevant to the molecular mechanisms involved in Jatropha seed maturation. These data can also be utilized regarding other Euphorbiaceae species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |