Unravelling the role of 17β-estradiol on advancing uterine luteolytic cascade in cattle
| Autor: | Claudia Maria Bertan Membrive, Guilherme Pugliesi, Mario Binelli, Angela Maria Gonella-Diaza, I.R. Feltrin, M. L. Oliveira, Aurea M O Canavessi, T. Martins, S. C. Scolari, B. P. Mello, Roberto Sartori |
|---|---|
| Přispěvatelé: | State University of Maranhão Tocantine Region, Universidade de São Paulo (USP), University of Florida, Universidade Estadual Paulista (UNESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Prostaglandin Luteolysis VACAS Luteal phase Endometrium Placebo Dinoprost chemistry.chemical_compound Endocrinology Food Animals Corpus Luteum Internal medicine medicine Animals Progesterone Estrous cycle Estradiol business.industry Uterus Oxytocin receptor Estrogen medicine.anatomical_structure chemistry Receptors Oxytocin Animal Science and Zoology Cattle Female business |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Made available in DSpace on 2022-04-28T19:43:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In cattle, 17β-estradiol (E2) stimulates prostaglandin F2α (PGF2α) synthesis, which causes luteolysis. Except for the well-established upregulation of oxytocin receptor gene (OXTR), molecular mechanisms of E2-induced PGF2α release in vivo remain unknown. We hypothesized that E2-induced PGF2α release requires de novo transcription of components of the PGF2α synthesis machinery. Beef cows (n = 52) were assigned to remain untreated (Control; n = 10), to receive 50% ethanol infusion intravenously (Placebo; n = 21), or 3 mg E2 in 50% ethanol infusion intravenously (Estradiol; n = 21) on day 15 (D15) after estrus. We collected a single endometrial biopsy per animal at the time of the treatment (0h; Control B0h group), 4 hours (4h; Placebo B4h group and Estradiol B4h group), or 7 hours (7h; Placebo B7h group and Estradiol B7h group) post-treatment. Compared to the Placebo group, the Estradiol group presented significantly greater 13,14-dihydro-15-keto-PGF2α concentrations between 4h and 7h and underwent earlier luteolysis. At 4h, the qPCR analysis showed a lower abundance of ESR1, ESR2 and aldo-keto reductase family 1 member B1 (AKR1B1) genes in the Estradiol B4h group, and a greater abundance of OXTR compared to the Placebo B4h group. Similarly, the E2 treatment significantly reduced the abundance of AKR1B1, and AKR1C4 in the Estradiol B7h group, compared to the placebo group. Overall, E2-induced PGF2α release and luteolysis involved an unexpected and transient downregulation of components of the PGF2α-synthesis cascade, except for OXTR, which was upregulated. Collectively, our data suggest that E2 connects newly-synthesized OXTR to pre-existing cellular machinery to synthesize PGF2α and cause luteal regression. Agrarian Sciences Center State University of Maranhão Tocantine Region Department of Animal Reproduction University of São Paulo North Florida Research and Education Center Institute of Food and Agricultural Sciences University of Florida Department of Animal Sciences University of Florida Department of Pharmacology and Biotechnology São Paulo State University Department of Animal Science Luiz de Queiroz College of Agriculture University of São Paulo Department of Animal Sciences São Paulo State University Department of Pharmacology and Biotechnology São Paulo State University Department of Animal Sciences São Paulo State University FAPESP: 2015/03331-3) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|