Clonal growth of normal and malignant human breast epithelia
| Autor: | Sam Lan, Martha R. Stampfer, Helene S. Smith |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Pathology
medicine.medical_specialty Breast Neoplasms Cell Count In Vitro Techniques Biology medicine Humans Breast Fibroblast Cells Cultured Clonal growth Cancer Epithelial Cells General Medicine Fibroblasts medicine.disease Clone Cells Culture Media medicine.anatomical_structure Linear relationship Oncology Colony formation Female Surgery Human breast |
| Zdroj: | Journal of Surgical Oncology. 18:317-322 |
| ISSN: | 1096-9098 0022-4790 |
| DOI: | 10.1002/jso.2930180313 |
| Popis: | We have developed an efficient method for the growth of colonies from single breast epithelial cells derived from nonmalignant [6], primary [7], and metastatic [1] surgical specimens. The immediate sources of the cells were primary and secondary mass cultures. An enriched culture medium and fibroblast feeder layers were used. A linear relationship between the number of cells cultured and the number of colonies obtained was found. Efficiency of colony formation was higher for those derived from nonmalignant specimens (mean 24%) than those derived from malignant specimens (mean 11%). Fibroblast feeder layers increased the efficiency of colony formation and also supported extended growth of colonies. |
| Databáze: | OpenAIRE |
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