Photodegradation and phototoxicity studies of furosemide. Involvement of singlet oxygen in the photoinduced hemolysis and lipid peroxidation
| Autor: | Franklin Vargas, Ingert Martinez Volkmar, Julio Sequera, Jenny Rojas, Rafael Medina, Germán Fraile, Mait Velásquez, Hisbeth Mendez |
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| Rok vydání: | 1998 |
| Předmět: |
Erythrocytes
Photochemistry Biophysics chemistry.chemical_element Hemolysis Oxygen Lipid peroxidation chemistry.chemical_compound Furosemide medicine Humans Radiology Nuclear Medicine and imaging Diuretics Photodegradation Benzoic acid Radiation Singlet Oxygen Radiological and Ultrasound Technology Singlet oxygen Human serum albumin chemistry Hydroxyl radical Lipid Peroxidation Phototoxicity Dermatitis Phototoxic medicine.drug |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 42:219-225 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/s1011-1344(98)00074-8 |
| Popis: | The phototoxic diuretic drug furosemide (1), a 5-(aminosulfonyl)-4-chloro-2-[(2-furanylmethyl)-amino] benzoic acid is photolabile under aerobic and anaerobic conditions. Irradiation of a methanol solution of 1 under oxygen produces photoproducts 2, 3, 4 and singlet oxygen, while under argon the photoproducts 2 and 4 were isolated. A peroxidic unstable photoproduct was detected during the photolysis under oxygen atmosphere. The formation of singlet oxygen by photolysis of 1 was evidenced by trapping with 2,5-dimethylfuran (GC-mass), furfuryl alcohol and 1,3-cyclohexadiene-1,4-diethanoate (HPLC) as 1O2 scavengers and by the histidine test. Furosemide was screened in vitro at different concentrations for UV-Vis-induced phototoxic effects in a photohemolysis test, in the presence and absence of different radical scavengers, singlet oxygen and hydroxyl radical quenchers. However, furosemide photosensitized the peroxidation of linoleic acid, as monitored by the UV-detection of dienic hydroperoxides and it also photosensitized the oxidation of histidine. The photodegradation was catalyzed in the presence of human serum albumin. Studies on peripheral blood mononuclear and polymorphonuclear cells (lymphocytes and neutrophils) demonstrated no phototoxicity on these cell lines. |
| Databáze: | OpenAIRE |
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