Proteomic analysis of Lactobacillus pentosus for the identification of potential markers of adhesion and other probiotic features
| Autor: | Natacha Caballero Gómez, Esther Alonso, Antonio Gálvez, Nabil Benomar, Beatriz Pérez Montoro, Charles W. Knapp, Hikmate Abriouel, Saïd Ennahar, Peter Horvatovich |
|---|---|
| Přispěvatelé: | Area de Microbiologıia, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén (UJA), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Dpt Civil, Environmental & Architectural Engineering, University of Kansas, University of Kansas [Lawrence] (KU), Analytical Biochemistry, Medicinal Chemistry and Bioanalysis (MCB) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Protein moonlighting Proteomics SURFACE Swine 030106 microbiology BIFIDOBACTERIUM Lactobacillus pentosus In Vitro Techniques Phosphoglycerate mutase 03 medical and health sciences MOONLIGHTING PROTEINS Heat shock protein Gene expression PLANTARUM [CHIM]Chemical Sciences Animals KEY PROTEINS GE FERMENTATION Probiotics Mucin BIOFILM FORMATION Mucins Biofilm qRT-PCR Mucus MICROBIAL-POPULATIONS 030104 developmental biology Biochemistry HUMAN PLASMINOGEN Food Microbiology Adhesion Biomarkers Food Science |
| Zdroj: | Food Research International Food Research International, Elsevier, 2018, 111, pp.58-66. ⟨10.1016/j.foodres.2018.04.072⟩ Food Research International, 111, 58-66. Elsevier |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2018.04.072⟩ |
| Popis: | International audience; We analyzed the adhesion capacity to mucus of 31 Lactobacillus pentosus strains isolated from naturally fermented Aloreña green table olives using an immobilized mucin model. On the basis of their adhesive capacity to mucin, three phenotypes were selected for cell-wall protein proteomic analysis to pinpoint proteins involved in the adhesion process: the highly adhesive L. pentosus CF1-43 N (73.49% of adhesion ability), the moderately adhesive L. pentosus CF1-37 N (49.56% of adhesion ability) and the poorly adhesive L. pentosus CF2-20P (32.79% of adhesion ability). The results revealed four moonlighting proteins over-produced in the highly adhesive L. pentosus CF1-43 N, which were under/not produced in the other two L. pentosus strains (CF1-37 N and CF2-20P). These proteins were involved in glycolytic pathway (phosphoglycerate mutase and glucosamine-6-phosphate deaminase), stress response (small heat shock protein) and transcription (transcription elongation factor GreA). Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of the genes coding for these four moonlighting proteins in the highly adhesive L. pentosus CF1-43 N versus the poorly adhesive L. pentosus CF2-20P and also in response to mucin for 20 h which clearly indicate the significant role of these genes in the adhesion capacity of L. pentosus. Thus, these proteins could be used as biomarkers for mucus adhesion in L. pentosus. On the other hand, mucin exposure induced other probiotic effects in L. pentosus strains, enhancing their co-aggregation ability with pathogens and possible inactivation. |
| Databáze: | OpenAIRE |
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