Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis
| Autor: | Koki Uchida, Minako Suzuki, Tadaaki Nakajima, Yasuhiro Tomooka, Tomomi Okumura, Y. Ikeda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
primary culture
Cell type animal structures biology QH301-705.5 Patinopecten yessoensis Marine invertebrates biology.organism_classification Embryonic stem cell General Biochemistry Genetics and Molecular Biology Cell biology scallop stomatognathic system Cell culture Scallop Hepatopancreas genetic transfection Biology (General) Adductor muscles gonadal cells Research Articles Research Article |
| Zdroj: | FEBS Open Bio, Vol 11, Iss 8, Pp 2282-2291 (2021) FEBS Open Bio |
| ISSN: | 2211-5463 |
| Popis: | Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines. We developed a primary culture system for female and male gonad‐, hepatopancreas‐, and adductor muscle‐derived cells from Japanese scallop, Patinopecten yessoensis. In the female gonadal cell culture, we succeeded in maintaining the cultured cells for at least one month and the culture had potential for cell proliferation. We attempted transfection of female gonadal cells with scallop‐derived promoters and lipofection reagent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text