A pericellular hyaluronan matrix is required for the morphological maturation of cortical neurons
| Autor: | Shinji Miyata, Tsukasa Matsuda, Kenzi Oshima, Mina Takechi, Hiroshi Kitagawa, Daita Nadano |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Neurite Neurogenesis Biophysics Matrix (biology) Biochemistry Extracellular matrix Mice 03 medical and health sciences 0302 clinical medicine Neuroplasticity medicine Animals Hyaluronic Acid Molecular Biology Cells Cultured HAS1 Cerebral Cortex Neurons Mice Inbred ICR biology Embryonic stem cell Extracellular Matrix Cell biology Hyaluronan synthase 030104 developmental biology medicine.anatomical_structure Cerebral cortex biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1864:129679 |
| ISSN: | 0304-4165 |
| Popis: | Background Hyaluronan (HA) is a major component of the extracellular matrix (ECM) and is involved in many cellular functions. In the adult brain, HA forms macromolecular aggregates around synapses and plays important roles in neural plasticity. In contrast to the well-characterized function of HA in the adult brain, its roles in the developing brain remain largely unknown. Methods Biochemical and histochemical analyses were performed to analyze the amount, solubility, and localization of HA in the developing mouse brain. By combining in utero labeling, cell isolation, and in vitro cultures, we examined the expression of hyaluronan synthase (HAS) and morphological maturation of cortical neurons. Results The amount of HA increased during perinatal development and decreased in the adult. HA existed as a soluble form in the early stages; however, its solubility markedly decreased during postnatal development. HA localized in cell-sparse regions in the embryonic stages, but was broadly distributed during the postnatal development of the cerebral cortex. Developing cortical neurons expressed both Has2 and Has3, but not Has1, suggesting the autonomous production of HA by neurons themselves. HA formed a pericellular matrix around the cell bodies and neurites of developing cortical neurons, and the inhibition of HA synthesis reduced neurite outgrowth. Conclusion The formation of the pericellular HA matrix is essential for the proper morphological maturation of developing neurons. General significance This study provides new insights into the roles of hyaluronan in the brain. development. |
| Databáze: | OpenAIRE |
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