The effect of endotoxin-contaminated medium on in vitro fertilization and development of bovine oocytes matured in vitro
| Autor: | B. Avery, R. Høier, T. Wamberg, V. Madison, V. Mortensen, M. Balle, Torben Greve |
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| Přispěvatelé: | Revues Inra, Import |
| Jazyk: | francouzština |
| Rok vydání: | 1991 |
| Předmět: |
Lipopolysaccharides
Male Embryology medicine.medical_specialty Zygote medicine.medical_treatment Parthenogenesis Medicine (miscellaneous) Fertilization in Vitro Andrology Embryonic and Fetal Development Oogenesis [SDV.BDD] Life Sciences [q-bio]/Development Biology medicine Animals Blastocyst Bovine serum albumin Cells Cultured Limulus Test [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology Gynecology In vitro fertilisation biology Embryogenesis Serum Albumin Bovine Embryo Polyspermy Spermatozoa Sperm In vitro Culture Media Endotoxins [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition medicine.anatomical_structure Reproductive Medicine Fertilization Oocytes biology.protein Cattle Female Animal Science and Zoology Developmental Biology Food Science |
| Popis: | The purpose of this study was to determine whether the presence of bacterial endotoxin in the bovine serum albumin (BSA) used to supplement media utilized for sperm preparation and co-culture of bovine sperm and oocytes affects in vitro penetration and embryonic development of oocytes matured in vitro. The chromogenic limulus amoebocyte lysate (LAL) test was used for quantification of the content of endotoxin. The proportion of penetrated ova was significantly greater (P greater than 0.0005) for the endotoxin-contaminated group (89%) versus the non-contaminated group (61%), but this was probably not due to endotoxin contamination. The presence of endotoxin resulted in a high rate of polyspermy (27% versus 4%, respectively; P greater than 0.0005), while the occurrence of parthenogenetic activation was the same for each group (8%). The proportion of total embryos put into culture that developed to the blastocyst stage by day 8 was similar (30% and 26%) for the contaminated and non-contaminated group, respectively. Fifty-three and 69%, respectively, hatched on day 10. These results suggest that endotoxin induces polyspermy, but has no adverse effect on embryonic development. |
| Databáze: | OpenAIRE |
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