Dietary sesamin suppresses aortic NADPH oxidase in DOCA salt hypertensive rats
| Autor: | Daisuke Kurumazuka, Yasuo Matsumura, Yoshinobu Kiso, Akira Nishiyama, Yukiko Nagai, Daisuke Nakano |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Physiology Dioxoles Lignans Cyclic N-Oxides Rats Sprague-Dawley chemistry.chemical_compound Sesamin Physiology (medical) Internal medicine medicine Animals Lucigenin Desoxycorticosterone Aorta Pharmacology Lignan NADPH oxidase biology Superoxide NOX4 NADPH Oxidases Rats Endocrinology chemistry Biochemistry NOX1 Dietary Supplements Hypertension cardiovascular system biology.protein Spin Labels P22phox |
| Zdroj: | Clinical and experimental pharmacologyphysiology. 35(3) |
| ISSN: | 1440-1681 |
| Popis: | 1. Dietary sesamin, a sesame lignan, is known to suppress the development of experimental hypertension in rats partly through its inhibitory effect on vascular O(2)(-) production. Therefore, in the present study, we examined whether sesamin feeding had any effect on vascular NADPH oxidase using aortas from deoxycorticosterone acetate (DOCA) salt hypertensive rats. 2. After a 5 week feeding and treatment period, aortic O(2)(-) production and NADPH oxidase activity were measured using the lucigenin assay. Reverse transcription-polymerase chain reaction was performed to analyse aortic expression of NADPH oxidase subunit (p22phox, gp91phox, Nox1 and Nox4) mRNA. 3. Sesamin feeding markedly suppressed DOCA salt-induced hypertension and significantly decreased aortic O(2)(-) production. DOCA salt treatment increased NADPH oxidase activity and elevated aortic mRNA expression of p22phox, gp91phox, Nox1 and Nox4. Sesamin feeding abolished the increase in NADPH oxidase activity and, furthermore, significantly suppressed increases in p22phox, gp91phox and Nox1 mRNA expression. 4. In conclusion, dietary sesamin prevented DOCA salt-induced increases in NADPH oxidase activity and subunit mRNA expression. These effects seem to be involved in the anti-oxidant and antihypertensive effects of sesamin. |
| Databáze: | OpenAIRE |
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