Digital quantification of DNA by mapping polarization degree related with coding gold nanorods
| Autor: | Di Zu, Wenlan Liu, Shuqing Sun, Xiangyu Sun, Zhenjie Wu, Hui Tan, Ran Liao |
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| Rok vydání: | 2017 |
| Předmět: |
Hepatitis B virus
Materials science Light Monte Carlo method 02 engineering and technology 010402 general chemistry 01 natural sciences Light scattering symbols.namesake Optics Dispersion (optics) Scattering Radiation Stokes parameters Electrical and Electronic Engineering Surface plasmon resonance Engineering (miscellaneous) Nanotubes Linear polarization business.industry Surface Plasmon Resonance 021001 nanoscience & nanotechnology Polarization (waves) Atomic and Molecular Physics and Optics 0104 chemical sciences DNA Viral Microscopy Electron Scanning symbols Nanorod Gold DNA Probes 0210 nano-technology business Monte Carlo Method Software |
| Zdroj: | Applied Optics. 56:9301 |
| ISSN: | 2155-3165 1559-128X |
| Popis: | The development of highly sensitive and low-cost methods for detecting DNA is of critical importance. Here, we describe a strategy for the highly sensitive and low-cost digital detection of target DNA. Individual DNA molecules were encoded with a single gold nanorod (Au NR), which was then separated and enriched using the magnetic immune-separation process, followed by dehybridization and dispersion into a buffer solution and immobilization on glass slides for polarized dark-field microscopic imaging. With the imaging we can get the first three data sets of the Stokes vector, and the experimental degree of the linear polarization of the light scattered by the Au NR was obtained. Using the Monte Carlo simulation program, the Muller matrix of the Au NRs was simulated and the simulated degree of the linear polarization was calculated to be 0.58. Based on the experimental and simulated degree of the linear polarization, the Au NRs were identified and quantified with an in-house Matlab program, and the concentration of the target DNA at the femtomolar level was therefore achieved. |
| Databáze: | OpenAIRE |
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