Quantitative studies of phytohemagglutinin-induced DNA and RNA synthesis in normal and agammaglobulinemic leukocytes
| Autor: | Douglass C. Tormey, H. Hugh Fudenberg, Roberta M. Kamin |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Acquired Agammaglobulinemia
Immunoassay medicine.diagnostic_test Immunology RNA DNA Biology RNA chemical synthesis Molecular biology In vitro Article chemistry.chemical_compound chemistry Agammaglobulinemia hemic and lymphatic diseases Culture Techniques Lectins medicine Leukocytes Immunology and Allergy Humans |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Popis: | Leukocytes from nine patients with acquired agammaglobulinemia were studied in vitro. Synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) induced by phytohemagglutinin was measured by determination of the degree of incorporation of labeled precursor. Synthesis of both DNA and RNA was decreased in the agammaglobulinemic cells. The presence of an inhibitor in the patients' sera could not be demonstrated. These results suggest that the basic defect in agammaglobulinemia is cellular rather than humoral. |
| Databáze: | OpenAIRE |
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