Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
| Autor: | Hirohisa Mekata, Yukie Katayama, Mai Shiokawa, Mai Kishimoto, Tetsuo Asai, Saki Kokawa, Shinobu Tsuchiaka, Tetsuya Mizutani, Tatsushi Morita, Hiroshi Aoki, Tsutomu Omatsu, Shinji Makino, Sayed Samim Rahpaya, Atsushi Kobayashi, Keiko Otsu, Nariaki Nonaka, Yoshiteru Murata, Ayako Hasebe, Tamaki Okabayashi, Mami Oba, Moeko Umetsu, Yuka Nunomura, Yumi Kirino, Ahmad Jan Abi, Takashi Kimura, Tomohiro Yamaguchi |
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| Rok vydání: | 2018 |
| Předmět: |
Diarrhea
0301 basic medicine Disease reservoir Insecta 040301 veterinary sciences Respiratory Tract Diseases virulence factors Cattle Diseases Rodentia Disease Vectors Real-Time Polymerase Chain Reaction Virus law.invention Birds 0403 veterinary science 03 medical and health sciences law medicine Animals Pathogen Polymerase chain reaction General Veterinary biology Transmission (medicine) fungi disease reservoirs 04 agricultural and veterinary sciences Abortion Veterinary biology.organism_classification Virology Dembo polymerase chain reaction Neospora caninum 030104 developmental biology cattle Salmonella enterica Original Article medicine.symptom |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| Popis: | Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time. |
| Databáze: | OpenAIRE |
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