Grape powder consumption affects the expression of neurodegeneration-related brain proteins in rats chronically fed a high-fructose-high-fat diet
| Autor: | Jung Su Chang, Chi Hao Wu, Liang Mao Chou, Shyh Hsiang Lin, Ching I. Lin, Yi Wen Chien, Hsiang Liao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Male medicine.medical_specialty NF-E2-Related Factor 2 Endocrinology Diabetes and Metabolism Clinical Biochemistry Receptor for Advanced Glycation End Products tau Proteins Fructose Biology Diet High-Fat Biochemistry 03 medical and health sciences 0302 clinical medicine Glycation Internal medicine medicine Amyloid precursor protein Animals Vitis Cognitive decline Rats Wistar Receptor Molecular Biology Brain-derived neurotrophic factor Nutrition and Dietetics Kinase Brain-Derived Neurotrophic Factor Body Weight Brain Neurodegenerative Diseases Metabolism 030104 developmental biology Endocrinology Hyperglycemia Dietary Supplements biology.protein Powders Cognition Disorders 030217 neurology & neurosurgery |
| Zdroj: | The Journal of nutritional biochemistry. 43 |
| ISSN: | 1873-4847 |
| Popis: | Abnormal glucose metabolism in the brain is recognized to be associated with cognitive decline. Because grapes are rich in polyphenols that produce antioxidative and blood sugar-lowering effects, we investigated how grape consumption affects the expression and/or phosphorylation of neurodegeneration-related brain proteins in aged rats fed a high-fructose-high-fat (HFHF) diet. Wistar rats were maintained on the HFHF diet from the age of 8 weeks to 66 weeks, and then on an HFHF diet containing either 3% or 6% grape powder as an intervention for 12 weeks. Western blotting was performed to measure the expression/phosphorylation levels of several cortical and hippocampal proteins, including amyloid precursor protein (APP), tau, phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated kinase (ERK), receptor for advanced glycation end products (RAGEs), erythroid 2-related factor 2 (Nrf2) and brain-derived neurotrophic factor (BDNF). Inclusion of up to 6% grape powder in the diet markedly reduced RAGE expression and tau hyperphosphorylation, but upregulated the expression of Nrf2 and BDNF, as well as the phosphorylation of PI3K and ERK, in the brain tissues of aged rats fed the HFHF diet. Thus, grape powder consumption produced beneficial effects in HFHF-diet-fed rats, exhibiting the potential to ameliorate changes in neurodegeneration-related proteins in the brain. |
| Databáze: | OpenAIRE |
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