Methylglyoxal and Advanced Glycation End products: Insight of the regulatory machinery affecting the myogenic program and of its modulation by natural compounds
Autor: | Inho Choi, Jin Yeul Ma, Jalaluddin M. Ashraf, Gulam Rabbani, Eun Ju Lee, Arif Tasleem Jan, Khurshid Ahmad, Won-Kyung Cho, Yong-Ho Lee, Mohammad Hassan Baig, Taeyeon Kim, Han Sol Min |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
Glycation End Products
Advanced 0301 basic medicine medicine.medical_specialty Curcumin Science Receptor for Advanced Glycation End Products Catechols Myostatin Muscle Development MyoD Article Cell Line Diabetes Mellitus Experimental RAGE (receptor) 03 medical and health sciences chemistry.chemical_compound Glycation Internal medicine medicine Animals Computer Simulation RNA Messenger Myogenin Biological Products Multidisciplinary biology Myogenesis Gingerol Methylglyoxal Cell Differentiation Pyruvaldehyde musculoskeletal system Mice Inbred C57BL 030104 developmental biology Endocrinology Gene Expression Regulation chemistry Biochemistry Gene Knockdown Techniques biology.protein Medicine Fatty Alcohols |
Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) SCIENTIFIC REPORTS(7) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Methylglyoxal (MG) is a reactive dicarbonyl intermediate and a precursor of advanced glycation end products (AGEs). The authors investigated the role played by AGEs in muscle myopathy and the amelioration of its effects by curcumin and gingerol. In addition to producing phenotypical changes, MG increased oxidative stress and reduced myotube formation in C2C12 cells. RAGE (receptor for AGEs) expression was up-regulated and MYOD and myogenin (MYOG) expressions were concomitantly down-regulated in MG-treated cells. Interestingly, AGE levels were higher in plasma (~32 fold) and muscle (~26 fold) of diabetic mice than in control mice. RAGE knock-down (RAGEkd) reduced the expressions of MYOD and MYOG and myotube formation in C2C12 cells. In silico studies of interactions between curcumin or gingerol and myostatin (MSTN; an inhibitor of myogenesis) and their observed affinities for activin receptor type IIB (ACVRIIB) suggested curcumin and gingerol reduce the interaction between MSTN and ACVRIIB. The findings of this study suggest enhanced AGE production and subsequent RAGE-AGE interaction obstruct the muscle development program, and that curcumin and gingerol attenuate the effect of AGEs on myoblasts. |
Databáze: | OpenAIRE |
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