Tumor-induced VEGF-C overexpression in retroperitoneal lymph nodes in VX2 carcinoma-bearing rabbits
| Autor: | Yan Ling Feng, Yin Wang, Yun Zhou, Rong Zhen Luo, Jihong Liu, Chun Yan Lan, Yong Wen Huang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Retroperitoneal Lymph Node Vascular Endothelial Growth Factor C Pharmaceutical Science Uterine Cervical Neoplasms VEGF-C cisplatin Antineoplastic Agents Real-Time Polymerase Chain Reaction Drug Discovery medicine Retroperitoneal space Animals RNA Messenger Retroperitoneal Space Lymph node Original Research Pharmacology Cisplatin Drug Design Development and Therapy VX2 business.industry peripheral blood Immunohistochemistry Tumor Burden Gene Expression Regulation Neoplastic medicine.anatomical_structure Real-time polymerase chain reaction Vascular endothelial growth factor C Lymphatic Metastasis Disease Progression Female retroperitoneal lymph nodes Lymph Rabbits business medicine.drug |
| Zdroj: | Drug Design, Development and Therapy |
| ISSN: | 1177-8881 |
| Popis: | Yong-Wen Huang,1,* Yun Zhou,1,* Chun-Yan Lan,1 Yin Wang,1 Yan-Ling Feng,1 Rong-Zhen Luo,2 Ji-Hong Liu1 1Department of Gynecology, 2Department of Pathology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in SouthChina, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People’s Republic of China *These authors contributed equally tothis work Objective: To establish the retroperitoneal lymph node (RLN) metastasis model of cervical carcinoma in rabbits and evaluate the relationship of vascular endothelial growth factor-C (VEGF-C) expression and the lymph node status. Methods: Forty-eight rabbits were injected with VX2 cells or RPMI solution at muscular mucosae of the myometrium 0.5 cm away from the cervix. Animals were treated with or without cis-diamminedichloroplatinum(II) (cisplatin: DDP) and sacrificed on days 15, 21, and 27 post-VX2 or RPMI injections. Tumor mass and RLNs were examined histopathologically. Quantitative real-time PCR was used to examine the changes in VEGF-C mRNA expression. Levels of VEGF-C protein expression in tissues were determined using immunohistochemistry staining. Results: Development of VX2 cervical carcinoma and the RLNs metastasis was confirmed with pathological examination. Significantly increased tumor volume was observed on days 15, 21, and 27 postinjection (P |
| Databáze: | OpenAIRE |
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