Weak power frequency magnetic fields induce microtubule cytoskeleton reorganization depending on the epidermal growth factor receptor and the calcium related signaling

Autor: Mei-Ping Cao, Wei-Tao Song, Juan Du, Xia Wu, Ruohong Xia, Shu-De Chen
Rok vydání: 2018
Předmět:
0301 basic medicine
Cell signaling
Physiology
Intracellular Space
lcsh:Medicine
Signal transduction
Biochemistry
Microtubules
Fluorophotometry
Ion Channels
Spectrum Analysis Techniques
Fluorescence Resonance Energy Transfer
Medicine and Health Sciences
Epidermal growth factor receptor
Post-Translational Modification
Phosphorylation
Enzyme Inhibitors
lcsh:Science
Cytoskeleton
Calcium signaling
Multidisciplinary
biology
Chemistry
Physics
Cell migration
Cell biology
Electrophysiology
ErbB Receptors
Cell Motility
Spectrophotometry
Physical Sciences
Cellular Structures and Organelles
EGFR signaling
Intracellular
Research Article
Calcium Channels
L-Type
Biophysics
Neurophysiology
Cell Migration
Research and Analysis Methods
Cell Line
03 medical and health sciences
Electromagnetic Fields
Microtubule
Humans
Calcium Signaling
lcsh:R
Biology and Life Sciences
Proteins
030104 developmental biology
Quinazolines
biology.protein
lcsh:Q
Calcium
Calcium Channels
Developmental Biology
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 10, p e0205569 (2018)
ISSN: 1932-6203
Popis: We have shown previously that a weak 50 Hz magnetic field (MF) invoked the actin-cytoskeleton, and provoked cell migration at the cell level, probably through activating the epidermal growth factor receptor (EGFR) related motility pathways. However, whether the MF also affects the microtubule (MT)-cytoskeleton is still unknown. In this article, we continuously investigate the effects of 0.4 mT, 50 Hz MF on the MT, and try to understand if the MT effects are also associated with the EGFR pathway as the actin-cytoskeleton effects were. Our results strongly suggest that the MF effects are similar to that of EGF stimulation on the MT cytoskeleton, showing that 1) the MF suppressed MT in multiple cell types including PC12 and FL; 2) the MF promoted the clustering of the EGFR at the protein and the cell levels, in a similar way of that EGF did but with higher sensitivity to PD153035 inhibition, and triggered EGFR phosphorylation on sites of Y1173 and S1046/1047; 3) these effects were strongly depending on the Ca2+ signaling through the L-type calcium channel (LTCC) phosphorylation and elevation of the intracellular Ca2+ level. Strong associations were observed between EGFR and the Ca2+ signaling to regulate the MF-induced-reorganization of the cytoskeleton network, via phosphorylating the signaling proteins in the two pathways, including a significant MT protein, tau. These results strongly suggest that the MF activates the overall cytoskeleton in the absence of EGF, through a mechanism related to both the EGFR and the LTCC/Ca2+ signaling pathways.
Databáze: OpenAIRE
Externí odkaz: