The hydrophobic segment ofArabidopsis thalianacluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes
| Autor: | F. Guerbette, Eric Ruelland, Catherine Cantrel, Alain Zachowski, Dominique Çiçek, Chantal Vergnolle, Susanne Bolte, Marie-Noëlle Vaultier, Yohann Boutté |
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| Přispěvatelé: | Université Pierre et Marie Curie - Paris 6 (UPMC), Umeå University, Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Diacylglycerol Kinase Molecular Sequence Data Arabidopsis Biophysics [SDV.BC]Life Sciences [q-bio]/Cellular Biology Endoplasmic Reticulum GFP 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound DIACYLGLYCEROL KINASES Structural Biology Genetics Arabidopsis thaliana Amino Acid Sequence INTEGRAL PROTEIN Phosphatidylinositol Molecular Biology Integral membrane protein Conserved Sequence 030304 developmental biology Diacylglycerol kinase MEMBRANE SEQUESTERING 0303 health sciences biology Endoplasmic reticulum ARABIDOPSIS THALIANA Intracellular Membranes Cell Biology Phosphatidic acid HYDROPHOBIC DOMAIN biology.organism_classification Protein Structure Tertiary Cell biology Membrane chemistry Hydrophobic and Hydrophilic Interactions 010606 plant biology & botany |
| Zdroj: | FEBS Letters FEBS Letters, 2008, 582 (12), pp.1743-1748. ⟨10.1016/j.febslet.2008.04.042⟩ FEBS Letters, Wiley, 2008, 582 (12), pp.1743-1748. ⟨10.1016/j.febslet.2008.04.042⟩ |
| ISSN: | 0014-5793 1873-3468 |
| Popis: | International audience; Diacylglycerol kinases (DGKs) catalyze the phosphorylation of diacylglycerol into phosphatidic acid. To fulfill their role in many signalling processes, DGKs must be located at, or in, membranes. Most mammalian DGKs are cytosolic and are recruited to membranes upon stimulation, except for e type DGKs that are permanently membrane-associated through a hydrophobic segment. Nothing is known about the mechanism(s) involved in the membrane localization of plant DGKs. By fusion to fluorescent proteins, we show that two DGKs from cluster I in Arabidopsis thaliana possess amino-terminal hydrophobic segments that are sufficient to address them to endoplasmic reticulum membranes. |
| Databáze: | OpenAIRE |
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