Method to Quantify Cytokines and Chemokines in Mouse Brain Tissue Using Bio-Plex Multiplex Immunoassays
| Autor: | Dorian B. McGavern, Brett Houser, Qian Gao, Amy Hendricksen, Kenneth J. Oh, Rejane Rua, Woei Tan, Daniel Braunschweig, Monica Manglani |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Analyte
Chemokine Plasmodium berghei medicine.medical_treatment Malaria Cerebral Enzyme-Linked Immunosorbent Assay Brain tissue General Biochemistry Genetics and Molecular Biology Article Proinflammatory cytokine 03 medical and health sciences Mice medicine Animals Humans Multiplex Molecular Biology Bio plex 030304 developmental biology 0303 health sciences biology medicine.diagnostic_test Chemistry 030302 biochemistry & molecular biology Brain Microspheres High-Throughput Screening Assays Disease Models Animal Cytokine Biochemistry Immunoassay biology.protein Cytokines Biological Assay Chemokines Biomarkers |
| Popis: | This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex. |
| Databáze: | OpenAIRE |
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