Viral-bacterial (co-)occurrence in the upper airways and the risk of childhood pneumonia in resource-limited settings

Autor: Blandina T. Mmbaga, Christa E. van der Gaast-de Jongh, Linda Minja, James S. Ngocho, Marien I. de Jonge, Janette Rahamat-Langendoen, Jeroen D. Langereis
Rok vydání: 2020
Předmět:
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
030106 microbiology
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Tanzania
Virus
Article
law.invention
03 medical and health sciences
All institutes and research themes of the Radboud University Medical Center
0302 clinical medicine
Community-acquired pneumonia
Childhood pneumonia
law
Internal medicine
Nasopharynx
Medicine
Humans
030212 general & internal medicine
Respiratory system
Child
Polymerase chain reaction
business.industry
Infant
Pneumonia
medicine.disease
Community-Acquired Infections
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Infectious Diseases
Real-time polymerase chain reaction
Case-Control Studies
Child
Preschool
business
Limited resources
Zdroj: J Infect
Journal of Infection, 81, 213-220
Journal of Infection, 81, 2, pp. 213-220
ISSN: 1532-2742
0163-4453
Popis: Objective To examine the association between bacterial-viral co-occurrence in the nasopharynx and the risk of community acquired pneumonia (CAP) in young children living in resource-limited settings. Methods A case-control study was conducted between January and December 2017 in Moshi, Tanzania. Children 2–59 months with CAP and healthy controls were enrolled. RSV and Influenza A/B were detected with a standardized polymerase chain reaction (PCR) method, and a simplified real-time quantitative PCR method, without sample pre-processing, was developed to detect bacterial pathogens in nasopharyngeal samples. Results A total of 109 CAP patients and 324 healthy controls were enrolled. Co-detection of H. influenzae and S. pneumoniae in nasopharyngeal swabs was linked with higher odds of CAP (aOR=3.2, 95% CI=1.1–9.5). The majority of the H. influenzae isolated in cases and controls (95.8%) were non-typeable. Of the viruses examined, respiratory syncytial virus (RSV) was most common (n = 31, 7.2%) in cases and controls. Children with RSV had 8.4 times higher odds to develop pneumonia than healthy children (aOR=8.4, 95%CI= 3.2 – 22.1). Conclusions Co-occurence of H. influenzae and S. pneumoniae in the nasopharynx was strongly associated with CAP. The high prevalence of non-typeable H. influenzae might be a sign of replacement as a consequence of Haemophilus influenzae type b vaccination.
Databáze: OpenAIRE
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