Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress

Autor: Francisco R. Tadeo, Daniel Gonzalez-Ibeas, Concha Domingo, Daniel Ventimilla, Manuel Talon
Rok vydání: 2020
Předmět:
Zdroj: BMC Plant Biology, Vol 20, Iss 1, Pp 1-14 (2020)
BMC Plant Biology
ISSN: 1471-2229
Popis: BackgroundIDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plantArabidopsis thaliana(Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of theIDA-like andHAE-like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploidNicotiana benthamiana,in order to identify members involved in abscission, stem growth and in the response to drought conditions.ResultsThe phylogenetic relationships among theIDA-like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, althoughNbenIDA2Alacked the hormonal regulatory elements. Expression analyses showed that the pair ofNbenIDA1homeologs were upregulated during corolla abscission.NbenIDA1andNbenIDA2pairs showed tissue differential expression under water stress conditions, sinceNbenIDA1homeologs were highly expressed in stressed leaves whileNbenIDA2homeologs, especiallyNbenIDA2B, were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of theIDA-like family and their putativeHAE-like receptors.ConclusionOur results suggest that the pair ofNbenIDA1homeologs are involved in the natural process of corolla abscission while both pairs ofNbenIDA1andNbenIDA2homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae.
Databáze: OpenAIRE
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