14-3-3 γ-isoform detection distinguishes sporadic Creutzfeldt–Jakob disease from other dementias
| Autor: | J Boons, Patrick Cras, B Van Everbroeck |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Blotting Western Short Report Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Creutzfeldt-Jakob Syndrome Central nervous system disease Diagnosis Differential Cerebrospinal fluid Western blot False positive paradox Medicine Humans Antiserum biology medicine.diagnostic_test business.industry medicine.disease Psychiatry and Mental health 14-3-3 Proteins Polyclonal antibodies biology.protein Surgery Neurology (clinical) Human medicine Antibody Differential diagnosis business |
| Zdroj: | Journal of neurology, neurosurgery and psychiatry |
| ISSN: | 0022-3050 |
| Popis: | We developed a polyclonal antiserum directed to the gamma-isoform of the human 14-3-3 protein and compared the immunoreactivity with a commercially available antibody (CG31). We analysed 14-3-3 in 253 cerebrospinal fluid samples blinded for the diagnosis by western blot and ELISA, with a commonly used polyclonal antiserum (Sc-731) and the c specific antibodies. Our patient population consisted of 52 patients with definite sporadic Creutzfeldt - Jakob disease (sCJD) and 201 patients with a different final diagnosis. We obtained similar sensitivity, ranging from 96% to 98% with all antibodies. Of all the samples that were false positive with Sc-731, 50% were negative with both gamma-isoform specific antibodies resulting in a significantly higher specificity (85% v 93%, respectively). If only sCJD and patients with dementia differing from sCJD were analysed we found that 64% of false positives were negative which also resulted in significantly increased specificity and positive predictive value. The gamma-isoform specific antibodies strongly improve the specificity of the immunoblot and might improve worldwide acceptance of the use of the 14-3-3 assay in the differential diagnosis of sCJD. |
| Databáze: | OpenAIRE |
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