Stability of toluene oxidation by Pseudomonas putida under nutrient deprivation

Autor: R. O. Jenkins, S. C. Heald
Rok vydání: 1996
Předmět:
Zdroj: Applied Microbiology and Biotechnology. 46:388-392
ISSN: 1432-0614
0175-7598
DOI: 10.1007/bf00166234
Popis: Toluene-induced cells of Pseudomonas putida NCIMB 11767 lost their ability to oxidise toluene within 300 h under conditions of carbon/energy or nitrogen deprivation at 30°C, while incubation at 4°C improved the stability of this activity. Provision of inducing substrates (toluene or phenol) to nitrogen-deprived cells at 30°C also enhanced the stability of toluene oxidation, whereas provision of a non-inducing carbon/energy source (ethanol) led to a total loss of toluene oxidation within 160 h. Disappearance of toluene-induced proteins, at different rates accompanied the loss of toluene oxidation in carbon-deprived cells. The data suggest that degradation of one or more of the major proteins of toluene metabolism determines the stability of toluene oxidation in carbon-deprived cells. Around 40% of the whole-cell toluene oxidation rate was recoverable after cryopreservation (−20°C under glycerol) of toluene-induced cells but most of this recovered activity (86%) was associated with dead cells. These observations may have important implications for the application of these toluene-induced cells as in situ bioremediation catalysts.
Databáze: OpenAIRE
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