Fabrication, Characterization and Cellular Compatibility of Poly(Hydroxy Alkanoate) Composite Nanofibrous Scaffolds for Nerve Tissue Engineering

Autor: Clemens van Blitterswijk, J. Hilderink, Mohammad Hossein Nasr-Esfahani, Elahe Masaeli, Saeid Sadri, Lorenzo Moroni, Aart A. van Apeldoorn, Mohammad Morshed
Přispěvatelé: Developmental BioEngineering, Faculty of Science and Technology
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Anatomy and Physiology
Nanofibers
lcsh:Medicine
Gene Expression
Biocompatible Materials
Biochemistry
Engineering
Tissue engineering
Molecular Cell Biology
Materials Testing
Fiber
Nerve Tissue
lcsh:Science
chemistry.chemical_classification
Neurons
Multidisciplinary
3-Hydroxybutyric Acid
Tissue Scaffolds
Chemistry
IR-84574
Neurochemistry
Polymer
Electrospinning
Membrane
Cellular Types
Type I collagen
Research Article
Biotechnology
Neurogenesis
Materials Science
Biomedical Engineering
Bioengineering
Neurological System
Biomaterials
Differential scanning calorimetry
Developmental Neuroscience
METIS-294969
Neuroglial Development
Prohibitins
Animals
Biology
Cell Proliferation
Tissue Engineering
lcsh:R
technology
industry
and agriculture
Rats
Chemical engineering
nervous system
Nanofiber
lcsh:Q
Schwann Cells
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, 8(2):e57157, 1-13. Public Library of Science
PLoS ONE, Vol 8, Iss 2, p e57157 (2013)
ISSN: 1932-6203
Popis: Tissue engineering techniques using a combination of polymeric scaffolds and cells represent a promising approach for nerve regeneration. We fabricated electrospun scaffolds by blending of Poly (3-hydroxybutyrate) (PHB) and Poly (3-hydroxy butyrate-co-3- hydroxyvalerate) (PHBV) in different compositions in order to investigate their potential for the regeneration of the myelinic membrane. The thermal properties of the nanofibrous blends was analyzed by differential scanning calorimetry (DSC), which indicated that the melting and glass temperatures, and crystallization degree of the blends decreased as the PHBV weight ratio increased. Raman spectroscopy also revealed that the full width at half height of the band centered at 1725 cm(-1) can be used to estimate the crystalline degree of the electrospun meshes. Random and aligned nanofibrous scaffolds were also fabricated by electrospinning of PHB and PHBV with or without type I collagen. The influence of blend composition, fiber alignment and collagen incorporation on Schwann cell (SCs) organization and function was investigated. SCs attached and proliferated over all scaffolds formulations up to 14 days. SCs grown on aligned PHB/PHBV/collagen fibers exhibited a bipolar morphology that oriented along the fiber direction, while SCs grown on the randomly oriented fibers had a multipolar morphology. Incorporation of collagen within nanofibers increased SCs proliferation on day 14, GDNF gene expression on day 7 and NGF secretion on day 6. The results of this study demonstrate that aligned PHB/PHBV electrospun nanofibers could find potential use as scaffolds for nerve tissue engineering applications and that the presence of type I collagen in the nanofibers improves cell differentiation.
Databáze: OpenAIRE
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