Cumulus Cells Are Potential Candidates for Cell Therapy
| Autor: | Fikrettin Şahin, Oya Akcin, Cem Ficicioglu, Eslam Essam Mohammed, Barbaros Nalbantoğlu, Esra Aydemir Çoban, Sema Yilmaz |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Cell- and Tissue-Based Therapy Down-Regulation Apoptosis General Biochemistry Genetics and Molecular Biology Cell therapy Downregulation and upregulation Pancreatic cancer Cell Line Tumor medicine Biomarkers Tumor Humans Pharmacology chemistry.chemical_classification Reactive oxygen species Cumulus Cells biology Gene Expression Profiling CD44 fungi Cell cycle Oocyte medicine.disease Cell biology Pancreatic Neoplasms medicine.anatomical_structure Hyaluronan Receptors chemistry biology.protein Oocytes Research Article |
| Zdroj: | In vivo (Athens, Greece). 33(6) |
| ISSN: | 1791-7549 |
| Popis: | Background/Aim: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells. Materials and Methods: CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days. Results: Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle. Conclusion: CCs conditioned medium induced apoptosis of pancreatic cancer cells. |
| Databáze: | OpenAIRE |
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