Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro
| Autor: | Hai-Ying Lei, Xiu-Ying Cai, Yu-Ying Shen, Qing Cai, Shuang-Qing Chen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Time Factors Combined use Immunofluorescence Green fluorescent protein Mice Developmental Neuroscience Neural Stem Cells Neurotrophic factors Internal medicine Glial Fibrillary Acidic Protein Nerve Growth Factor medicine Animals Nerve Growth Factors reproductive and urinary physiology Cells Cultured Cell Proliferation medicine.diagnostic_test Chemistry Brain-Derived Neurotrophic Factor Significant difference Brain Nestin Embryo Mammalian In vitro Neural stem cell Fibroblast Growth Factors Mice Inbred C57BL Drug Combinations Endocrinology nervous system Phosphopyruvate Hydratase cardiovascular system Neuroscience Developmental Biology |
| Zdroj: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 38 |
| ISSN: | 1873-474X |
| Popis: | Neurotrophic factors can promote the proliferation and differentiation of neural stem cells (NSCs). Here we report that the possibility of using bFGF in combination with BDNF and NGF to promote proliferation and differentiation of NSCs in vitro. C57BL/6 mouse NSCs were cultured, passaged and stained by immunofluorescence for nestin and GFP. According to different neurotrophic factors added to NSCs, seven experiment groups (NGF, BDNF, bFGF, bFGF+NGF, bFGF+BDNF, NGF+BDNF and NGF+BDNF+bFGF) and a blank control group were established. One week after induction and differentiation, results showed that there was significant difference in the percentage of NSCs differentiating into neurons among the experiment groups. The percentage in the multi-factor groups was significantly higher than that in the single-factor groups (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text