Transcription arrest relief by S-II/TFIIS during gene expression in erythroblast differentiation
| Autor: | Kazuhisa Sekimizu, Makiko Nagata, Nagisa Arimitsu, Hiroshi Koyama, Takahiro Ito |
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| Rok vydání: | 2009 |
| Předmět: |
Erythroblasts
Transcription Genetic Gene Expression RNA polymerase II beta-Globins Histones Mice Sp3 transcription factor Genetics Animals Enhancer Regulation of gene expression Mice Knockout General transcription factor biology Promoter Acetylation Cell Differentiation Cell Biology TCF4 Molecular biology Introns Liver TAF2 biology.protein RNA Polymerase II Transcriptional Elongation Factors |
| Zdroj: | Genes to cells : devoted to molecularcellular mechanisms. 14(3) |
| ISSN: | 1365-2443 |
| Popis: | Transcription stimulator S-II relieves RNA polymerase II (RNAPII) from transcription elongation arrest. Mice lacking the S-II gene (S-II KO mice) die at mid-gestation with impaired erythroblast differentiation, and have decreased expression of the Bcl-x gene. To understand a role of S-II in Bcl-x gene expression, we examined the distribution of transcription complex on the Bcl-x gene in S-II KO mice. The amount of RNAPII at intron 2 of the Bcl-x gene was decreased in S-II KO mice, whereas recruitment of transcription initiation factor TFIIB and RNAPII to the promoter was not decreased. Consistently, in vitro transcription analysis revealed the presence of a transcription arrest site in the Bcl-x gene intron 2, and transcription arrest at this site was overcome by S-II. Furthermore, histone acetylation on the coding region of the Bcl-x gene was decreased in S-II KO mice. In the beta(major)-globin gene, whose expression was also decreased in S-II KO mice, there were no changes in RNAPII distribution or histone acetylation, but the amount of histone H3 occupying the coding region was increased. These results suggest that S-II is involved in transcription of the Bcl-x and beta(major)-globin gene during erythroblast differentiation, by relieving transcription arrest or affecting histone modification on chromatin template. |
| Databáze: | OpenAIRE |
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