Multimodal Positron Emission Tomography Imaging to Quantify Uptake of 89Zr-Labeled Liposomes in the Atherosclerotic Vessel Wall
| Autor: | Erik S.G. Stroes, Carlos Pérez-Medina, Sean Carlin, Zahi A. Fayad, Andreas Kjaer, Jason Bini, Julia Witjes, Chang Ho Wessel, Luuk Giesen, Francois Fay, Tina Binderup, Thomas Reiner, Mark E. Lobatto, Philip M. Robson, Gert Storm, Willem J. M. Mulder, Mootaz Eldib, Samantha Baxter, Jason S. Lewis, Claudia Calcagno |
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| Přispěvatelé: | Precision Medicine, ICMS Core, Biomaterials Science and Technology, Radiology and Nuclear Medicine, Graduate School, Vascular Medicine, ACS - Atherosclerosis & ischemic syndromes, ACS - Diabetes & metabolism, AGEM - Digestive immunity, AGEM - Endocrinology, metabolism and nutrition, Afd Pharmaceutics, Pharmaceutics |
| Rok vydání: | 2019 |
| Předmět: |
Male
Noninvasive imaging Biodistribution UT-Hybrid-D Biomedical Engineering Pharmaceutical Science Bioengineering Vascular permeability 02 engineering and technology 01 natural sciences Article In vivo medicine Animals Tissue Distribution Aorta Abdominal Radioisotopes Pharmacology Liposome medicine.diagnostic_test 010405 organic chemistry Chemistry business.industry Organic Chemistry 22/2 OA procedure Magnetic resonance imaging Atherosclerosis 021001 nanoscience & nanotechnology Plaque Atherosclerotic 0104 chemical sciences Positron emission tomography Positron-Emission Tomography Liposomes Rabbits Zirconium 0210 nano-technology Nuclear medicine business Ex vivo Biotechnology |
| Zdroj: | Bioconjugate Chemistry, 31(2), 360-368. American Chemical Society Bioconjugate chemistry, 31(2), 360-368. American Chemical Society Bioconjugate Chemistry, 31(2), 360. American Chemical Society : Division of Carbohydrate Chemistry Bioconjug Chem |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/acs.bioconjchem.9b00256 |
| Popis: | Nanotherapy has recently emerged as an experimental treatment option for atherosclerosis. To fulfill its promise, robust noninvasive imaging approaches for subject selection and treatment evaluation are warranted. To that end, we present here a positron emission tomography (PET)-based method for quantification of liposomal nanoparticle uptake in the atherosclerotic vessel wall. We evaluated a modular procedure to label liposomal nanoparticles with the radioisotope zirconium-89 (89Zr). Their biodistribution and vessel wall targeting in a rabbit atherosclerosis model was evaluated up to 15 days after intravenous injection by PET/computed tomography (CT) and PET/magnetic resonance imaging (PET/MRI). Vascular permeability was assessed in vivo using three-dimensional dynamic contrast-enhanced MRI (3D DCE-MRI) and ex vivo using near-infrared fluorescence (NIRF) imaging. The 89Zr-radiolabeled liposomes displayed a biodistribution pattern typical of long-circulating nanoparticles. Importantly, they markedly accumulated in atherosclerotic lesions in the abdominal aorta, as evident on PET/MRI and confirmed by autoradiography, and this uptake moderately correlated with vascular permeability. The method presented herein facilitates the development of nanotherapy for atherosclerotic disease as it provides a tool to screen for nanoparticle targeting in individual subjects' plaques. |
| Databáze: | OpenAIRE |
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