The T Allele of the 677C>T Polymorphism of Methylenetetrahydrofolate Reductase Gene is Associated With an Increased Risk of Ischemic Stroke in Polish Children
| Autor: | Ewa Emich-Widera, Beata Sarecka-Hujar, Janusz Wendorff, Elzbieta Marszał, Iwona Zak, Joanna Jachowicz-Jeszka, Ilona Kopyta |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Parents medicine.medical_specialty Hyperhomocysteinemia Adolescent Single-nucleotide polymorphism Polymerase Chain Reaction Polymorphism Single Nucleotide Gastroenterology Brain Ischemia Young Adult Gene Frequency Internal medicine Genotype Genetic variation Odds Ratio medicine Humans Genetic Predisposition to Disease Allele Child Allele frequency Methylenetetrahydrofolate Reductase (NADPH2) Genetics Sex Characteristics biology business.industry Sequence Analysis DNA Odds ratio medicine.disease Stroke Methylenetetrahydrofolate reductase Pediatrics Perinatology and Child Health biology.protein Female Poland Neurology (clinical) business |
| Zdroj: | Journal of Child Neurology. 24:1262-1267 |
| ISSN: | 1708-8283 0883-0738 |
| Popis: | Ischemic stroke is a very rare and multifactorial disease in children. The aim of the study was to analyze the relationship between the methylenetetrahydrofolate reductase (MTHFR) 677C>T polymorphism and stroke in Polish children and to observe whether there is any significant transmission of MTHFR alleles from heterozygous parents to their affected offspring. We analyzed 64 patients with stroke, 122 parents, and 59 healthy children. The MTHFR polymorphism was genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism. The T allele was more frequent in the stroke group (38%) than in controls (25%, P = .029, odds ratio = 1.84). We also found higher frequency of T allele in male patients compared to male controls (46% vs. 25%, P = .009, odds ratio = 2.53). The number of T allele carriers was again more prevalent in boys with stroke (71%) than in healthy boys (45%, P = .023, odds ratio = 3.09). The T allele was significantly transmitted in male patients (P < .019). We conclude that the MTHFR 677C>T polymorphism may be considered as a genetic risk factor of childhood stroke, especially in boys. |
| Databáze: | OpenAIRE |
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