Analysis of the impact of cryopreservation and theophylline on motility of sperm
Autor: | Hamid Shafi, Mir Mehrdad Farsi, Elaheh Gorji, Soraya Khafri |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
lcsh:R5-920 endocrine system 030219 obstetrics & reproductive medicine lcsh:QH471-489 urogenital system Ejaculation Testicular biopsy Obstetrics and Gynecology Motility Biology Sperm Cryopreservation Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine medicine lcsh:Reproduction Theophylline lcsh:Medicine (General) reproductive and urinary physiology Sperm motility medicine.drug |
Zdroj: | Middle East Fertility Society Journal, Vol 23, Iss 2, Pp 98-102 (2018) |
ISSN: | 1110-5690 |
DOI: | 10.1016/j.mefs.2017.09.002 |
Popis: | Objective: Sperm parameters, particularly motility, decrease during cryopreservation. Theophylline generally enhances sperm motility. We analyzed effects of theophylline and freezing on sperm motility.Design: Experimental study.Setting: Private IVF lab.Setting: IVF lab of Mehrgan Hospital. Method: 22–55 year-old men participated in this study (30 fresh ejaculation and 8 TESE samples). After sperm analysis, we added theophylline (40 mM) to half of our samples as case group to compare motility with the remaining samples as control group. Cryopreservation was performed in two groups. After thawing, motility of both groups was recorded. Furthermore, theophylline (40 mM) was applied to both groups after thawing again. Result: After adding theophylline, sperm motility improved significantly in all samples. Sperm motility reduced in control group more than the study group after freeze-thaw procedure (P |
Databáze: | OpenAIRE |
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