Secondary metabolite production by cereal-associated penicillia during cultivation on cereal grains

Autor:	Jens Christian Frisvad, Kristian Fog Nielsen, Magnus Hallas-Møller
Rok vydání:	2018
Předmět:	0301 basic medicine food.ingredient Secondary Metabolism Food Contamination Biology Secondary metabolite Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound food medicine Yeast extract Agar Food science Mycotoxin Triticum Inoculation Penicillium food and beverages Hordeum General Medicine Mycotoxins biology.organism_classification Yeast Culture Media 030104 developmental biology chemistry Chemotaxonomy Food Microbiology Edible Grain Biotechnology medicine.drug
Zdroj:	Applied Microbiology and Biotechnology. 102:8477-8491
ISSN:	1432-0614 0175-7598
DOI:	10.1007/s00253-018-9213-0
Popis:	Cereals are vulnerable substrates for fungal growth and subsequent mycotoxin contamination. One of the major fungal genera to colonize the ecosystem of stored grain is Penicillium, especially species in the series of Viridicata and Verrucosa. Culturing these species on grains, we hoped to induce the production of relevant secondary metabolites produced by these fungi in the early stage of cereal breakdown. In a multivariate setup six different cereal grains (wheat, rye, barley, oat, rice, and maize), one kind of white beans, and two standard fungal media, Yeast Extract Sucrose agar (YES agar) and Czapek Yeast Autolysate agar (CYA agar), were inoculated with the ten most important cereal-associated species from Penicillium (P. aurantiogriseum, P. cyclopium, P. freii, P. melanoconidium, P. neoechinulatum, P. polonicum, P. tricolor, P. viridicatum, P. hordei, and P. verrucosum). P. nordicum is a meat-associated species, which was included due to its chemical association with P. verrucosum, in addition to see if a substrate change would alter the profile of known chemistry. We found that cereals function very well as substrates for secondary metabolite production, but did not present significantly different secondary metabolite profiles, concerning known chemistry, as compared to standard laboratory agar media. However, white beans altered the semi-quantitative secondary metabolite profiles for several species. Correlations between substrates and certain metabolites were observed, as illuminated by principal component analysis. Many bioactive secondary metabolites were observed for the first time in the analyzed fungal species, including ergot type alkaloids in P. hordei.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50aff2452d0c030b0c957c63e821e5c3 https://doi.org/10.1007/s00253-018-9213-0 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.