Two transcripts from the rotund region of Drosophila show similar positional specificities in imaginal disc tissues
| Autor: | M Agnel, Ruth Griffin-Shea, C Vola, S Kerridge |
|---|---|
| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
MESH: Mutation
Transcription Genetic Immunoblotting medicine.disease_cause MESH: Drosophila melanogaster 03 medical and health sciences 0302 clinical medicine Gene mapping Drosophilidae Gene expression Genetics medicine Animals MESH: Animals MESH: Cloning Molecular Cloning Molecular Gene [SDV.BDD]Life Sciences [q-bio]/Development Biology Alleles 030304 developmental biology 0303 health sciences Mutation biology MESH: Immunoblotting MESH: Alleles MESH: Transcription Genetic Chromosome Mapping biology.organism_classification Molecular biology Phenotype MESH: Genes White (mutation) Imaginal disc MESH: Genetic Techniques Drosophila melanogaster Genes Genetic Techniques Larva MESH: DNA Probes DNA Probes MESH: Chromosome Mapping MESH: Larva 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Genes and Development Genes and Development, 1989, 3 (1), pp.85-95 Genes and Development, Cold Spring Harbor Laboratory Press, 1989, 3 (1), pp.85-95 |
| ISSN: | 0890-9369 |
| Popis: | International audience; The rotund (rn) mutation in Drosophila is unique in that its phenotype is limited to the deletion of specific distal parts, though not the extremities, of all adult appendages. We have cloned the rn gene, located at cytogenetic position 84D3,4, by chromosomal walking. The functional rn unit, defined genetically by the localization of 13 noncomplementing rn alleles, covers approximately 50 kb of DNA. Despite different developmental profiles, two transcript size classes (1.7 and 5.3 kb) from this region show an indistinguishable pattern of spatial expression in the imaginal discs at the white pupa stage. There is a high correlation between the specificity of the mutant phenotype and the accumulation of transcripts in the presumptive distal regions of the cuticle-forming epithelial cells of the affected discs; it is, in fact, the first gene reported whose expression is localized with respect to the proximo distal-forming axis. For both transcripts, we have also found, uniquely in the wing disc, expression limited to the anterior region of the mesodermally derived epithelial cells, which contribute to the muscles of the thorax. |
| Databáze: | OpenAIRE |
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