Discrimination of morphological findings in dentine from osteogenesis imperfecta patients using combinations of polarized light microscopy, microradiograhpy and scanning electron microscopy
| Autor: | Kari Storhaug, Wolfram Dietz, Ted Lundgren, Jörgen G. Norén, B. Lindau |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Adolescent Scanning electron microscope Dentinogenesis imperfecta Dentistry stomatognathic system Microscopy medicine Humans Child General Dentistry Aged Permanent teeth Polarized light microscopy business.industry Decalcification Technique Dentine structure Middle Aged Osteogenesis Imperfecta medicine.disease Microradiography stomatognathic diseases Osteogenesis imperfecta type I Osteogenesis imperfecta Child Preschool Dentin Microscopy Electron Scanning Microscopy Polarization business |
| Zdroj: | International Journal of Paediatric Dentistry. 9:253-261 |
| ISSN: | 1365-263X 0960-7439 |
| DOI: | 10.1111/j.1365-263x.1999.00143.x |
| Popis: | Objectives. The aim of this study was to investigate the morphological appearance of dentine in teeth from individuals with osteogenesis imperfecta type I, III and IV using different histological techniques, and to correlate morphological findings to different types of osteogenesis imperfecta. Sample and methods. Extracted or exfoliated primary and permanent teeth were collected from 15 patients with the osteogenesis imperfecta diagnoses I, III or IV, with or without the additional diagnosis dentinogenesis imperfecta. Ground and decalcified sections were prepared from the teeth. Histo-morphological studies of the dentine were performed utilizing light and polarized light microscopy, microradiography and scanning electron microscopy. Results. Characteristic findings were irregular tubules, remnants of capillary inclusions and obliterated pulps. All types of osteogenesis imperfecta exhibited similar types of dentine aberrations, but patients with type III or IV had a higher frequency of aberrations when compared to type I. Conclusions. The combination of either polarized light microscopy or micro-radiography, together with scanning electron microscopy, gave the most amount of morphological information from dentine samples. In addition, aberrations in dentine structure were more clearly observable. Light microscopy was not critical for the analyses. |
| Databáze: | OpenAIRE |
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