Hepatitis E outbreak in Jaipur due to Genotype IA
| Autor: | Deepa Meena, Farah Deba, Pratibha Sharma, Jitendra Tiwari, Aradhana Chouhan, Ruchi Singh, H N Verma, Bharti Malhotra, Khushbu Trivedi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male viruses lcsh:QR1-502 Scrub typhus medicine.disease_cause lcsh:Microbiology Disease Outbreaks 0302 clinical medicine Immunology and Microbiology (miscellaneous) Hepatitis E virus Pregnancy hev Immunology and Allergy 030212 general & internal medicine Child Phylogeny Reverse Transcriptase Polymerase Chain Reaction virus diseases Middle Aged Hepatitis E Infectious Diseases Child Preschool RNA Viral Female Viral hepatitis Microbiology (medical) Adult Adolescent Genotype 030106 microbiology Immunology viral hepatitis India Microbiology 03 medical and health sciences Young Adult medicine Humans Hepatitis Antibodies Serotyping Aged Hepatitis General Immunology and Microbiology outbreak business.industry water borne Outbreak medicine.disease Virology digestive system diseases Immunoglobulin M business Nested polymerase chain reaction Waste disposal |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 38, Iss 1, Pp 46-51 (2020) |
| ISSN: | 1998-3646 |
| Popis: | Purpose: Suddenly, many cases of fever with jaundice were reported from Sodala area at Jaipur. This outbreak of acute hepatitis at Jaipur Rajasthan was investigated for aetiology and subsequent phylogenetic analysis. Methods: Blood samples were collected from 106 symptomatic patients of acute hepatitis and 39 pregnant females (with or without symptoms of hepatitis) during an outbreak at Jaipur. The samples were tested for hepatitis A virus (HAV) and hepatitis E virus (HEV) by serological and molecular methods (polymerase chain reaction [PCR]). Sequencing of nested PCR product was done for phylogenetic analysis. Hepatitis B surface antigen (HBs antigen), anti-hepatitis C virus (HCV), anti-Leptospira and anti-scrub typhus IgM enzyme-linked immunosorbent assay (ELISA) was done for patients negative for HEV and HAV. Results: Among 106 symptomatic patients, HEV IgM was positive in 84/106 (79.2%) patients and HEV RNA in 72/106 (67.9%) patients. Among pregnant women, 6/39 (15.4%) were HEV IgM positive and 5/39 (12.8%) for HEV RNA. One (2.5%) pregnant woman died due to hepatitis. All the isolates belonged to genotype 1A of HEV. All HAV, HEV-negative samples were negative for HBs antigen, HCV antibody, Leptospira and scrub typhus IgM ELISA. Conclusion: The outbreak was due to HEV genotype 1A. The municipal water supply was contaminated and sanitary conditions and waste disposal were poor in the area. Boiling of drinking water, fixing the water supply pipes and frequent hand washing helped in controlling the outbreak. |
| Databáze: | OpenAIRE |
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