Antigen-specific stimulation and trans-stimulation of T cells in long-term culture
| Autor: | Andrei A. Augustin, Michael H. Julius, H. Cosenza |
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| Rok vydání: | 1979 |
| Předmět: |
Time Factors
Cell Survival T-Lymphocytes T cell Immunology Biology Lymphocyte Activation Epitope Epitopes Mice Antigen medicine Animals Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Cells Cultured Mice Inbred BALB C Mice Inbred C3H CD40 Molecular biology medicine.anatomical_structure Concanavalin A Polyclonal antibodies Hemocyanins biology.protein gamma-Globulins Chickens |
| Zdroj: | European Journal of Immunology. 9:665-670 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | When T cells from antigen-primed lymph nodes are stimulated in vitro with antigen, they give rise to a proliferative response as high as that elicited by the polyclonal T cell activator concanavalin A. It is likely that in these conditions not only antigen-specific T cells proliferate. We have established an experimental system which demonstrates that large numbers of nonantigen-specific T cells are induced to proliferate as a result of antigen-specific T cells' confrontation with antigen. This phenomenon, which we call trans-stimulation is antigen-dependent and antigen-specific. Although the presence of antigen-specific T cells is required, these cells do not have to proliferate in order to induce trans-stimulation. In an attempt to enrich for antigen-specific T cells in vitro, we established conditions for culturing T cells in the presence of antigen for long periods of time (up to 6 weeks). High levels of antigen reactivity were observed upon antigen restimulation of “directly” stimulated T cells from long-term cultures. In contrast, long-term cultures of trans-stimulated cells were depleted of antigen reactivity. Neither type of long-term culture contained detectable alloreactive cells indicating that trans-stimulation is not reflected randomly upon bystander T cells. |
| Databáze: | OpenAIRE |
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