Identification of peptide-binding sites within BSA using rapid, laser-induced covalent cross-linking combined with high-performance mass spectrometry
| Autor: | Melinda Hauser, Steven T. King, Chen Qian, Jeffrey M. Becker, Fred Naider, Robert L. Hettich, Sarah Kauffman |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Peptide Hormones Peptide Peptide binding Mass spectrometry Mass Spectrometry Pheromones Article 03 medical and health sciences Structural Biology Tandem Mass Spectrometry Fatty acid binding Animals Humans Binding site Bovine serum albumin Molecular Biology chemistry.chemical_classification Chromatography Binding Sites 030102 biochemistry & molecular biology biology Chemistry Serum Albumin Bovine 030104 developmental biology Biotinylation biology.protein Biophysics Cattle Peptides Avidin Protein Binding |
| Zdroj: | Journal of molecular recognition : JMR. 31(2) |
| ISSN: | 1099-1352 |
| Popis: | We are developing a rapid, time-resolved method using laser-activated cross-linking to capture protein-peptide interactions as a means to interrogate the interaction of serum proteins as delivery systems for peptides and other molecules. A model system was established to investigate the interactions between bovine serum albumin (BSA) and two peptides, the tridecapeptide budding-yeast mating pheromone (α-factor) and the decapeptide human gonadotropin-releasing hormone (GnRH). Cross-linking of α-factor, using a biotinylated, photoactivatable p-benzoyl-L-phenylalanine (Bpa) modified analog, was energy-dependent and achieved within seconds of laser irradiation. Protein blotting with an avidin probe was used to detect biotinylated species in the BSA-peptide complex. The cross-linked complex was trypsinized and then interrogated with nano-LC-MS/MS to identify the peptide cross-links. Cross-linking was greatly facilitated by Bpa in the peptide, but some cross-linking occurred at higher laser powers and high concentrations of a non-Bpa-modified α-factor. This was supported by experiments using GnRH, a peptide with sequence homology to α-factor, which was likewise found to be cross-linked to BSA by laser irradiation. Analysis of peptides in the mass spectra showed that the binding site for both α-factor and GnRH was in the BSA pocket defined previously as the site for fatty acid binding. This model system validates the use of laser-activation to facilitate cross-linking of Bpa-containing molecules to proteins. The rapid crosslinking procedure and high performance of MS/MS to identify cross-links provides a method to interrogate protein-peptide interactions in a living cell in a time-resolved manner. |
| Databáze: | OpenAIRE |
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