Biodistribution studies of ultrasmall silicon nanoparticles and carbon dots in experimental rats and tumor mice
| Autor: | Angélique Faramus, John L. Z. Ddungu, Ralf Bergmann, Garima Singh, Holger Stephan, Sebastian Hunoldt, Luisa De Cola, Constantin Mamat, Nadia Licciardello, Michele Maggini, Simone Silvestrini |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Silicon Biodistribution Nude Wistar Mice Nude Nanoparticle 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Experimental Mice chemistry.chemical_compound In vivo Neoplasms Quantum Dots Animals Tissue Distribution General Materials Science Surface charge Rats Wistar Bifunctional biodistribution copper-64 Chemistry Optical Imaging small animal positron emission tomography Carbon Copper Radioisotopes Female Nanoparticles Neoplasms Experimental Rats carbon quantum dots 021001 nanoscience & nanotechnology 0104 chemical sciences silicon nanoparticles Surface modification 0210 nano-technology Preclinical imaging Nuclear chemistry |
| Zdroj: | Nanoscale 10(2018), 9880-9891 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | Ultrasmall clearable nanoparticles possess enormous potential as cancer imaging agents. In particular, biocompatible silicon nanoparticles (Si NPs) and carbon quantum dots (CQDs) hold great potential in this regard. Their facile surface functionalization easily allows the introduction of different labels for in vivo imaging. However, to date, a thorough biodistribution study by in vivo positron emission tomography (PET) as well as a comparative study of Si vs C particles of similar size are missing. In this contribution, ultrasmall (size < 5 nm) Si NPs and CQDs were synthesized and characterized by high-resolution transmission electron microscopy (HR-TEM), Fourier-transform infrared (FTIR), absorption and steady-state emission spectroscopy. Subsequent functionalization of NPs with a near-infrared dye (Kodak-XS-670) or a radiolabel (64Cu) enabled a detailed in vitro and in vivo study of the particles. For radiolabeling experiments, the bifunctional chelating agent S-2-(4-Isothiocyanatobenzyl)-1,4,7-triaazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) was conjugated to the amino surface groups of the respective NPs. Efficient radiolabeling of NOTA-functionalized NPs with the positron emitter 64Cu was found. The biodistribution and PET studies showed a rapid renal clearance from the in vivo systems for both variants of the nanoparticles. Interestingly, the different derivatives investigated exhibited significant differences in the biodistribution and pharmacokinetic properties. This can mostly be attributed to different surface charge and hydrophilicity of the NPs, arising from the synthetic strategy used to prepare the particles. |
| Databáze: | OpenAIRE |
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